Product Details

Purity

>85% (SDS-PAGE)

Target Names

rpm-1

Uniprot No.

Q17551

Alternative Names

rpm-1; syd-3; C01B7.6; E3 ubiquitin-protein ligase rpm-1; EC 2.3.2.-; Pam/highwire/rpm-1 protein; Regulator of presynaptic morphology protein 1; Synapse defective protein 3

Species

Caenorhabditis elegans

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues. Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. Mediates ubiquitination and subsequent proteasomal degradation of target proteins, including dlk-1. Negatively regulates a p38 MAP kinase pathway composed of dlk-1, mkk-4, and pmk-3 that functions presynaptically to regulate synaptic architecture. Has a role during synaptogenesis; regulates neuronal morphology, specifically presynaptic differentiation. May regulate the spatial arrangement of presynaptic terminals or restrict their development. By targeting both dlk-1 and mlk-1 for degradation, may negatively regulate mlk-1/mek-1/kgb-1 and dlk-1/mkk-4/pmk-3 pathways which are involved in axon regeneration after injury. Regulates axon termination in PLM and ALM neurons. May play a role in the formation of muscle connections between the body wall and the motor axons in the dorsal and ventral cord.

Gene References into Functions

The findings provide insight into how growth cone collapse is regulated during axon termination in vivo, and suggest that RPM-1 signaling destabilizes microtubules to facilitate growth cone collapse and axon termination. PMID: 29084805
results indicate that RPM-1 uses different mechanisms to recruit FSN-1 and RAE-1 into independent signaling complexes in neurons PMID: 27979965
The findings show that axon termination defects are a core phenotype caused by loss of function in rpm-1. PMID: 24885325
RPM-1 negatively regulates a p38 MAP kinase pathway composed of the dual leucine zipper-bearing MAPKKK DLK-1, the MAPKK MKK-4, and the p38 MAP kinase PMK-3. PMID: 15707898
Our data suggest that RPM-1 positively regulates a Rab GTPase pathway to promote vesicular trafficking via late endosomes. PMID: 17698012
findings show that in mature neurons, RPM-1 resides in a distinct region that is close to, but does not overlap with, the synaptic exo- and endocytosis domains PMID: 18224716
RPM-1 regulates axon outgrowth affecting axon guidance and termination by controlling the trafficking of the UNC-5 and SAX-3 receptors to cell membranes. PMID: 18385318

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Subcellular Location

Cell projection, axon.

Protein Families

Highwire family

Tissue Specificity

Expression is seen in the pharynx, coelomocytes and distal tip cell. Most abundant expression is in axons of the nerve ring neuropil. Expression is not seen in body wall muscle, hypodermis or intestine.

Database Links

KEGG: cel:CELE_C01B7.6

STRING: 6239.C01B7.6

UniGene: Cel.2746

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Recombinant E3 ubiquitin-protein ligase rpm-1 (rpm-1), partial

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