RPT2 Antibody

Code CSB-PA727846XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPT2 Polyclonal antibody
Uniprot No.
Target Names
RPT2
Alternative Names
RPT2 antibody; At2g30520/At2g30510 antibody; T6B20.13/T6B20.14 antibody; Root phototropism protein 2 antibody; BTB/POZ domain-containing protein RPT2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RPT2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Signal transducer of the phototropic response and photo-induced movements. Necessary for root phototropism. Involved in hypocotyl phototropism under high rate but not under low rate light. Regulates stomata opening. Seems to be not involved in chloroplast accumulation and translocation.
Gene References into Functions
  1. Phototropic-responsive NPH3 family proteins NPH3 and RPT2 have roles in phototropin 1 (phot1) signaling besides phototropism. PMID: 28720608
  2. Here, we show that another phototropin-interacting NRL protein, NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1 (NCH1), and RPT2 redundantly mediate the chloroplast accumulation response but not the avoidance response PMID: 27578868
  3. Data conclude that photosensitivity is increased in the absence of RPT2 and that this results in the desensitization of phot1. PMID: 25873385
  4. RPT2 gene disruption does not affect blue light-dependent stomatal opening. PMID: 23352406
  5. RPT2 mediates true-leaf positioning and flattening. RPT2 function in the phot1 signaling pathway. PMID: 22739508
  6. Genetic analysis of RPT2 in Arabidopsis sheds additional light on the roles of the N- and C-terminal ends. PMID: 22836496
  7. Induction of RPT2 and reduction of gibberellin crucial for hypocotyl phototropic regulation by phy and cry. PMID: 20202166

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Protein Families
NPH3 family
Tissue Specificity
Expressed in hypocotyls, guard cells and mesophyll cells.
Database Links

KEGG: ath:AT2G30520

STRING: 3702.AT2G30520.1

UniGene: At.24430

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