Recombinant Arabidopsis thaliana Root phototropism protein 2 (RPT2), partial

Code CSB-YP727846DOA
MSDS
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Source Yeast
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Code CSB-EP727846DOA
MSDS
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Source E.coli
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Code CSB-EP727846DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP727846DOA
MSDS
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Source Baculovirus
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Code CSB-MP727846DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPT2
Uniprot No.
Alternative Names
RPT2; At2g30520/At2g30510; T6B20.13/T6B20.14; Root phototropism protein 2; BTB/POZ domain-containing protein RPT2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Signal transducer of the phototropic response and photo-induced movements. Necessary for root phototropism. Involved in hypocotyl phototropism under high rate but not under low rate light. Regulates stomata opening. Seems to be not involved in chloroplast accumulation and translocation.
Gene References into Functions
  1. Phototropic-responsive NPH3 family proteins NPH3 and RPT2 have roles in phototropin 1 (phot1) signaling besides phototropism. PMID: 28720608
  2. Here, we show that another phototropin-interacting NRL protein, NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1 (NCH1), and RPT2 redundantly mediate the chloroplast accumulation response but not the avoidance response PMID: 27578868
  3. Data conclude that photosensitivity is increased in the absence of RPT2 and that this results in the desensitization of phot1. PMID: 25873385
  4. RPT2 gene disruption does not affect blue light-dependent stomatal opening. PMID: 23352406
  5. RPT2 mediates true-leaf positioning and flattening. RPT2 function in the phot1 signaling pathway. PMID: 22739508
  6. Genetic analysis of RPT2 in Arabidopsis sheds additional light on the roles of the N- and C-terminal ends. PMID: 22836496
  7. Induction of RPT2 and reduction of gibberellin crucial for hypocotyl phototropic regulation by phy and cry. PMID: 20202166

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Protein Families
NPH3 family
Tissue Specificity
Expressed in hypocotyls, guard cells and mesophyll cells.
Database Links

KEGG: ath:AT2G30520

STRING: 3702.AT2G30520.1

UniGene: At.24430

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