Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRP6 Polyclonal antibody

Uniprot No.

Q12149

Target Names

RRP6

Alternative Names

RRP6 antibody; UNC733 antibody; YOR001W antibody; Exosome complex exonuclease RRP6 antibody; EC 3.1.13.- antibody; Ribosomal RNA-processing protein 6 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRP6 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Immunization of a rabbit with the recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRP6 protein stimulates its immune system to produce antibodies against the RRP6 protein. Once a sufficient amount of antibodies is generated, a serum sample is collected to obtain polyclonal antibodies. The RRP6 antibody undergoes protein A/G affinity chromatography purification. The effectiveness of the RRP6 antibody is assessed through ELISA and WB applications. This specific RRP6 antibody exhibits the ability to specifically react with the Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRP6 protein.

The Saccharomyces cerevisiae RRP6 protein mainly participates in the degradation and processing of various RNA molecules, which helps maintain the stability and integrity of the cellular RNA pool. RRP6 is a component of the exosome complex, which is involved in RNA surveillance and quality control. It plays a crucial role in the degradation of non-coding RNA, such as rRNA, tRNA, snRNA, and snoRNA. Additionally, RRP6 is also involved in the degradation of certain mRNA molecules.

Target Background

Function

Nuclear-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP6 has 3'-5' exonuclease activity which is not modulated upon association with Exo-9 suggesting that the complex inner RNA-binding path is not used to access its active site.

Gene References into Functions

Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, supporting the predicted widening of the lumen in the Rrp6-associated.Many exosome substrates exhibited clear preferences for a specific path to Rrp44. Both threading and direct access routes involve the RNA helicase Mtr4Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, suppor PMID: 28355211
Rrp6: Integrated roles in nuclear RNA metabolism and transcription termination PMID: 26612606
The data raise the intriguing possibility that Nab3 and Nrd1 can function independently to recruit Rrp6 to ncRNA targets, providing combinatorial flexibility in RNA processing. PMID: 25775092
The exosome-binding factors Rrp6 and Rrp47 form a composite surface for recruiting the Mtr4 helicase. PMID: 25319414
Rrp6 acts with the NNS complex(Nrd1, Nab3, and Sen1 ) globally to promote transcription termination in addition to 3' end RNA processing and/or degradation at specific targets. PMID: 25680078
3.3 A crystal structure of a ten-subunit RNA exosome complex from Saccharomyces cerevisiae composed of the Exo9 core and Rrp6 bound to single-stranded poly(A) RNA PMID: 25043052
Rrp47p reduces the exonucleolytic activity of Rrp6p on both single-stranded and structured RNA substrates without significantly altering the affinity towards RNA or the ability of Rrp6p to degrade RNA secondary structure. PMID: 24937447
results demonstrate that a principal function of Rrp47 is to facilitate appropriate expression levels of Rrp6. PMID: 24224060
In the nucleus of yeast cells, an eleventh component Rrp6 binds to the exosome, providing a second exoribonucleolytic site to the entire complex. PMID: 24189234
Rrp47 and Rrp6 have resolvable functions in Rrp6-mediated RNA surveillance and processing pathways. PMID: 24106327
Assembly of the yeast exoribonuclease Rrp6 with its associated cofactor Rrp47 occurs in the nucleus and is critical for the controlled expression of Rrp47 PMID: 23580640
Rrp6 plays a role in degrading distinct classes of meiotically induced noncoding RNAs during vegetative growth and the onset of meiosis. PMID: 21149693
Rrp6 is required for repair of UV-induced DNA damage PMID: 15489286
the major function of Rrp6p is in RNA surveillance PMID: 16260613
transcripts are somehow distinguished from true mRNAs and are degraded in an Rrp6p-dependent fashion in the nucleus PMID: 16484372
Nab6p binds poly(A)+ RNA, and the functions of Nab6p and Los1p suggest that mRNA metabolism and/or protein synthesis are growth rate limiting in rrp6Delta strains. PMID: 17101774
Results identify a domain within the yeast nucleolus that is enriched in polyadenylated RNAs in the absence of the nuclear exosome RNase Rrp6 or the exosome cofactor Mtr4. PMID: 17403903
Results suggest Rrp47p promotes Rrp6p activity by facilitating binding via the PMC2NT domain to structural elements within RNA. PMID: 17704127
The 3'-5' exonucleolytic domain of Rrp6p is necessary for establishing all quality control phenotypes associated with THO mutations. PMID: 18824516

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Subcellular Location

Nucleus, nucleolus.

Protein Families

Exosome component 10/RRP6 family

Database Links

KEGG: sce:YOR001W

STRING: 4932.YOR001W

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Code	CSB-PA619566XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: RRP6 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54.7 kDa Observed band size: 58 kDa
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RRP6 Antibody

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