Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RTE1 Polyclonal antibody

Uniprot No.

F4ITL6

Target Names

RTE1

Alternative Names

RTE1; At2g26070; T19L18.12; Protein REVERSION-TO-ETHYLENE SENSITIVITY1

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RTE1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts at an early step in the ethylene signaling pathway. Positively regulates ETR1, leading to the negative regulation of ethylene responses.

Gene References into Functions

the regulatory function of Arabidopsis RTH via its homolog RTE1 in the ethylene signaling pathway PMID: 28541511
Gene expression involved in ethylene signaling suppression was not reduced by the hpr1-5 mutation, which indicates selectivity of HPR1 for RTE1 expression affecting the consequent ethylene response. PMID: 25680185
Cb5 and RTE1 are functional partners in promoting ETR1-mediated repression of ethylene signaling. PMID: 24635651
The roles of CTR1 and RTE1 in ETR1 N-terminal signaling, were evaluated. PMID: 22566492
a high affinity association of RTE1 and ETR1 is important in the regulation of ETR1. PMID: 20952388
RTE1 is a negative regulator of ethylene signaling and suggest that RTE1 plays an important role in ETR1 function [RTE1]. PMID: 16682642
Our results suggest specificity of the RTE1 function to ETR1 and that endomembranes may play a role in the ethylene signal transduction. PMID: 17644624
we demonstrated the subcellular co-localization of RTE1 and ETR1. These findings support and enhance the genetic model that RTE1 plays a role in regulating ETR1. PMID: 17999643
RTE1 targets the ethylene-binding domain of ETR1 and positively regulates the ethylene receptor, independent of RAN1 activity. PMID: 18643990
Dominant etr1 mutations that require RTE1 to confer ethylene insensitivity are essentially silent when transferred to the identical conserved postitions in other ethylene receptor genes. PMID: 19369589

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.

Tissue Specificity

Strongly expressed in 1-4-day-old seedlings in the apical hook, cotyledons, root vascular tissue, root tip and root hairs, with little or no expression in the hypocotyl. In light-grown seedlings, expression could also be seen in the apex and young leaves,

Database Links

KEGG: ath:AT2G26070

STRING: 3702.AT2G26070.1

UniGene: At.28087

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RTE1 Antibody

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