sas-4 Antibody

Code CSB-PA333892XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans sas-4 Polyclonal antibody
Uniprot No.
Target Names
sas-4
Alternative Names
sas-4 antibody; F10E9.8Spindle assembly abnormal protein 4 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans sas-4 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for centrosome duplication. Plays a central role in determining centrosome size.
Gene References into Functions
  1. SAS-4 is a centriole-associated component whose amount dictates centrosome size PMID: 12600319
  2. SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication; then to the daughter centriole or a closely associated structure. SAS-4 recruitment is a key step in the centrosome duplication cycle. PMID: 12636923
  3. Centriolar SAS-4 remains in dynamic equilibrium with the cytoplasmic pool until late prophase, when it is stably incorporated in a step that requires gamma-tubulin and microtubule assembly. PMID: 18299348
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to the centrosome throughout the cell cycle. Localizes to a tiny dot in the center of centrosome. Recruited to the centrosome once per cell cycle, at the time of organelle duplication and remains stably associated after.
Database Links

KEGG: cel:CELE_F10E9.8

STRING: 6239.F10E9.8

UniGene: Cel.5956

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