Recombinant Spindle assembly abnormal protein 4 (sas-4), partial

Code CSB-YP333892CXY
MSDS
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Source Yeast
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Code CSB-EP333892CXY
MSDS
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Source E.coli
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Code CSB-EP333892CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333892CXY
MSDS
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Source Baculovirus
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Code CSB-MP333892CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sas-4
Uniprot No.
Alternative Names
sas-4; F10E9.8Spindle assembly abnormal protein 4
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for centrosome duplication. Plays a central role in determining centrosome size.
Gene References into Functions
  1. SAS-4 is a centriole-associated component whose amount dictates centrosome size PMID: 12600319
  2. SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication; then to the daughter centriole or a closely associated structure. SAS-4 recruitment is a key step in the centrosome duplication cycle. PMID: 12636923
  3. Centriolar SAS-4 remains in dynamic equilibrium with the cytoplasmic pool until late prophase, when it is stably incorporated in a step that requires gamma-tubulin and microtubule assembly. PMID: 18299348
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to the centrosome throughout the cell cycle. Localizes to a tiny dot in the center of centrosome. Recruited to the centrosome once per cell cycle, at the time of organelle duplication and remains stably associated after.
Database Links

KEGG: cel:CELE_F10E9.8

STRING: 6239.F10E9.8

UniGene: Cel.5956

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