Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SOBIR1 Polyclonal antibody

Uniprot No.

Q9SKB2

Target Names

SOBIR1

Alternative Names

SOBIR1 antibody; EVR antibody; At2g31880 antibody; F20M17.8 antibody; Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1 antibody; EC 2.7.10.1 antibody; EC 2.7.11.1 antibody; Protein EVERSHED antibody; Protein SUPPRESSOR OF BIR1-1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SOBIR1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death. Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity. Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission.

Gene References into Functions

AvrPto suppresses Arabidopsis SOBIR1-induced cell death in N. benthamiana. PMID: 28876174
BAK1 and SOBIR1 associate with each other in planta when the function of BIR1 is compromised. PMID: 27400831
SOBIR1 is involved in both penetration and post-penetration resistance to M. oryzae in Arabidopsis. PMID: 27023441
mutation of suppressor of BIR1-1, which encodes an emerging positive regulator of transmembrane receptors in plants, suppressed the effects of BAK1 overexpression. PMID: 25944825
AtSOBIR1 interacts with AtRLP23. PMID: 24525519
ER quality control plays important roles in the biogenesis of SOBIR1. PMID: 24498907
Functionality of ReMAX, much like that of related receptor-like proteins, depends on the presence of the receptor kinase SOBIR (SUPPRESSOR OF BIR1-1).[SOBIR] PMID: 24384530
RLP30, BAK1, and SOBIR1/EVR contribute to resistance against necrotrophic fungi. PMID: 24104566
Data show that SOBIR1/EVR is essential for receptor-like proteins (RLPs)-mediated immunity in planta. PMID: 23716655
SOBIR1 encodes a receptor-like kinase, probably functioning as a key regulator in a signal transduction pathway activated in bir1-1 mutant that constitutively activates defense responses, to promote cell death and disease resistance. [SOBIR1] PMID: 19616764

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Mostly present in leaves and flowers, with increasing expression in older flowers.

Database Links

KEGG: ath:AT2G31880

STRING: 3702.AT2G31880.1

UniGene: At.13945

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SOBIR1 Antibody

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