Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1 (SOBIR1), partial

Code CSB-YP865914DOA
MSDS
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Source Yeast
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Code CSB-EP865914DOA
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Source E.coli
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Code CSB-EP865914DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865914DOA
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Source Baculovirus
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Code CSB-MP865914DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SOBIR1
Uniprot No.
Alternative Names
SOBIR1; EVR; At2g31880; F20M17.8; Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1; EC 2.7.10.1; EC 2.7.11.1; Protein EVERSHED; Protein SUPPRESSOR OF BIR1-1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death. Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity. Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission.
Gene References into Functions
  1. AvrPto suppresses Arabidopsis SOBIR1-induced cell death in N. benthamiana. PMID: 28876174
  2. BAK1 and SOBIR1 associate with each other in planta when the function of BIR1 is compromised. PMID: 27400831
  3. SOBIR1 is involved in both penetration and post-penetration resistance to M. oryzae in Arabidopsis. PMID: 27023441
  4. mutation of suppressor of BIR1-1, which encodes an emerging positive regulator of transmembrane receptors in plants, suppressed the effects of BAK1 overexpression. PMID: 25944825
  5. AtSOBIR1 interacts with AtRLP23. PMID: 24525519
  6. ER quality control plays important roles in the biogenesis of SOBIR1. PMID: 24498907
  7. Functionality of ReMAX, much like that of related receptor-like proteins, depends on the presence of the receptor kinase SOBIR (SUPPRESSOR OF BIR1-1).[SOBIR] PMID: 24384530
  8. RLP30, BAK1, and SOBIR1/EVR contribute to resistance against necrotrophic fungi. PMID: 24104566
  9. Data show that SOBIR1/EVR is essential for receptor-like proteins (RLPs)-mediated immunity in planta. PMID: 23716655
  10. SOBIR1 encodes a receptor-like kinase, probably functioning as a key regulator in a signal transduction pathway activated in bir1-1 mutant that constitutively activates defense responses, to promote cell death and disease resistance. [SOBIR1] PMID: 19616764

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Mostly present in leaves and flowers, with increasing expression in older flowers.
Database Links

KEGG: ath:AT2G31880

STRING: 3702.AT2G31880.1

UniGene: At.13945

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