scube2 Antibody

Code CSB-PA705065XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) scube2 Polyclonal antibody
Uniprot No.
Target Names
scube2
Alternative Names
scube2 antibody; you antibody; Signal peptide antibody; CUB and EGF-like domain-containing protein 2 antibody; Protein You antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) scube2 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane. Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface. Synergizes with DISP1 to cause an increase in SHH secretion. Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis.
Gene References into Functions
  1. Emilin3 modulates the availability of hedgehog ligands by interacting with the permissive factor Scube2 in the notochord sheath PMID: 24131633
  2. Data show that knocking down the function of all three scube genes scube1, scube2 and scube3 simultaneously phenocopies a complete loss of Hedgehog (HH) signal transduction in the embryo. PMID: 22609552
  3. positional cloning and functional studies demonstrate that You is a novel extracellular component of the Hedgehog pathway in vertebrates(You gene) PMID: 15660164
  4. Scube2 can modulate the long-range action of Bmp-dependent Hedgehog signaling in the neural tube and somites. PMID: 15753045
  5. Scube2 may act during Hedgehog signal transduction prior to, or during, receipt of the Hedgehog signal at the plasma membrane PMID: 16626681

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Subcellular Location
Secreted. Cell surface.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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