Recombinant Danio rerio Signal peptide, CUB and EGF-like domain-containing protein 2 (scube2), partial

Code CSB-YP705065DIL
MSDS
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Source Yeast
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Code CSB-EP705065DIL
MSDS
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Source E.coli
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Code CSB-EP705065DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP705065DIL
MSDS
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Source Baculovirus
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Code CSB-MP705065DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
scube2
Uniprot No.
Alternative Names
scube2; you; Signal peptide; CUB and EGF-like domain-containing protein 2; Protein You
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane. Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface. Synergizes with DISP1 to cause an increase in SHH secretion. Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis.
Gene References into Functions
  1. Emilin3 modulates the availability of hedgehog ligands by interacting with the permissive factor Scube2 in the notochord sheath PMID: 24131633
  2. Data show that knocking down the function of all three scube genes scube1, scube2 and scube3 simultaneously phenocopies a complete loss of Hedgehog (HH) signal transduction in the embryo. PMID: 22609552
  3. positional cloning and functional studies demonstrate that You is a novel extracellular component of the Hedgehog pathway in vertebrates(You gene) PMID: 15660164
  4. Scube2 can modulate the long-range action of Bmp-dependent Hedgehog signaling in the neural tube and somites. PMID: 15753045
  5. Scube2 may act during Hedgehog signal transduction prior to, or during, receipt of the Hedgehog signal at the plasma membrane PMID: 16626681

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Subcellular Location
Secreted. Cell surface.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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