Sec13 Antibody

Code CSB-PA165513XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Sec13 Polyclonal antibody
Uniprot No.
Target Names
Sec13
Alternative Names
Sec13 antibody; CG6773 antibody; Protein SEC13 homolog antibody; GATOR complex protein SEC13 antibody; Secretory protein 13 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Sec13 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Recruited to transcriptionally active chromatin at the time of transcription initiation by RNA polymerase II. Required for proper expression of ecdysone-responsive genes such as Eip74EF and Eip75B during larval development. Required for reactivation of transcription after heat shock. Required for nuclear import of phosphorylated Mad via importin msk. Has no role in classical nuclear localization signal (cNLS)-dependent nuclear import via importin-beta.; A component of the GATOR subcomplex GATOR2 which functions as an activator of the amino acid-sensing branch of the TORC1 signaling pathway. The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation. GATOR2 activates the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex, controlling the switch to cell proliferation and growth under nutrient replete conditions and during female oocyte development.
Subcellular Location
Nucleus envelope. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, nuclear pore complex. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane. Note=Localizes to chromatin, specifically to areas undergoing transcriptional activation. Chromatin localization is independent of the nuclear pore complex (PubMed:20144761).
Protein Families
WD repeat SEC13 family
Tissue Specificity
Salivary glands.
Database Links

KEGG: dme:Dmel_CG6773

STRING: 7227.FBpp0083801

UniGene: Dm.7682

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