Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC2 Polyclonal antibody

Uniprot No.

P17065

Target Names

SEC2

Alternative Names

SEC2 antibody; YNL272C antibody; N0641Rab guanine nucleotide exchange factor SEC2 antibody; GDP-GTP exchange factor SEC2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exchange activity on YPT32.

Gene References into Functions

Yck1p and Yck2p phosphorylate sites within the Sec2p Ypt32p/Sec15p binding region and in doing so promote binding of Sec2p to Sec15p and inhibit binding to Ypt32p. PMID: 26700316
Structural investigation of the Sec4p-Sec2p complex reveals the role of the Sec2p coiled-coil domain in facilitating the fast kinetics of the exchange reaction. PMID: 26263895
The phosphorylation state of Sec2p can direct a switch in its regulatory binding partners that facilitates maturation of the secretory vesicle and helps to promote the directionality of vesicular transport. PMID: 24248333
Sec2p is normally released from the exocyst after vesicle tethering so that it can recycle onto a new round of vesicles. PMID: 16611746
The crystal structure of the Sec2p GEF domain at 3.0 A resolution was reported. PMID: 17292842
A hypothetical model of the exchange domain of sec2p as a pair of coiled-coil helices that binds to sec4p and facilitates nucleotide disassociation. PMID: 17346229

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Subcellular Location

Bud neck. Bud tip. Cytoplasmic vesicle, secretory vesicle. Note=Localizes to sites of polarized growth, namely to the bud neck and to the bud tip of growing buds, and associates with membranes. Colocalizes with secretory vesicles at exocytic sites. Proper localization is dependent on the actin cytoskeleton, MYO2, the kinesin-related protein SMY1, the Rab GTPase YPT32, and the production of post-Golgi vesicles.

Protein Families

SEC2 family

Database Links

KEGG: sce:YNL272C

STRING: 4932.YNL272C

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SEC2 Antibody

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Target Background