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Recombinant Saccharomyces cerevisiae Rab guanine nucleotide exchange factor SEC2 (SEC2), partial

Recombinant Saccharomyces cerevisiae Rab guanine nucleotide exchange factor SEC2 (SEC2), partial

Code	CSB-YP325476SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP325476SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP325476SVG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP325476SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP325476SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SEC2

Uniprot No.

P17065

Alternative Names

SEC2; YNL272C; N0641Rab guanine nucleotide exchange factor SEC2; GDP-GTP exchange factor SEC2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exchange activity on YPT32.

Gene References into Functions

Yck1p and Yck2p phosphorylate sites within the Sec2p Ypt32p/Sec15p binding region and in doing so promote binding of Sec2p to Sec15p and inhibit binding to Ypt32p. PMID: 26700316
Structural investigation of the Sec4p-Sec2p complex reveals the role of the Sec2p coiled-coil domain in facilitating the fast kinetics of the exchange reaction. PMID: 26263895
The phosphorylation state of Sec2p can direct a switch in its regulatory binding partners that facilitates maturation of the secretory vesicle and helps to promote the directionality of vesicular transport. PMID: 24248333
Sec2p is normally released from the exocyst after vesicle tethering so that it can recycle onto a new round of vesicles. PMID: 16611746
The crystal structure of the Sec2p GEF domain at 3.0 A resolution was reported. PMID: 17292842
A hypothetical model of the exchange domain of sec2p as a pair of coiled-coil helices that binds to sec4p and facilitates nucleotide disassociation. PMID: 17346229

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Subcellular Location

Bud neck. Bud tip. Cytoplasmic vesicle, secretory vesicle. Note=Localizes to sites of polarized growth, namely to the bud neck and to the bud tip of growing buds, and associates with membranes. Colocalizes with secretory vesicles at exocytic sites. Proper localization is dependent on the actin cytoskeleton, MYO2, the kinesin-related protein SMY1, the Rab GTPase YPT32, and the production of post-Golgi vesicles.

Protein Families

SEC2 family

Database Links

KEGG: sce:YNL272C

STRING: 4932.YNL272C

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