Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) secY Polyclonal antibody

Uniprot No.

P0AGA2

Target Names

secY

Alternative Names

Protein translocase subunit SecY secY prlA b3300 JW3262

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Synthesized peptide derived from internal of Escherichia coli (strain K12) secY protein (250-261aa )

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.

Gene References into Functions

These data suggest that wild-type YidC residues (G355 and M471) participate in interactions, direct or indirect, with SecY. PMID: 24187090
While translocation of loops 1 and 2 of TatC is strictly dependent on the Sec translocase and the YidC insertase, translocation of loop 3 does not depend on the translocase or insertase. PMID: 23058713
SecY signal peptide is found to specifically bind SecY translocase in a saturable manner and at levels comparable to those that stimulate SecA ATPase activity. PMID: 15476412
provide evidence for the direct interaction between FtsY and the SecY translocon PMID: 15815684
SecY plug domain moves away from the center of the channel toward SecE during polypeptide translocation, and the translocation-enhancing prlA3 mutation and SecG subunit change the properties of channel gating. PMID: 16148946
The data explain how conformational changes in SecA could be directly coupled to the previously proposed opening mechanism of the SecYEG channel. PMID: 16890955
One such translocation complex, SecY/Sec61, is found in every cell, in either the plasma membrane of bacteria and archaea or the endoplasmic reticulum membrane of eukaryotes. PMID: 17077865
the SecY plug contributes to the gating mechanism of the channel by maintaining the structure of the SecYEG complex in a compact closed state PMID: 17092931
normal SecY function is required for the folding of membrane proteins after their insertion into the translocon PMID: 17242069
study used purified E. coli components to address the mechanism of protein translocation; disulfide bridge crosslinking demonstrates that SecA transfers both the signal sequence and the mature region of a secreted substrate into a single SecY molecule PMID: 17418789
The resting cell membrane channel forms a barrier for small molecules, with both the pore ring and the plug required for the seal; channel opening requires movement of the plug. PMID: 17531809
Data suggest that SecY binds to the primary site on the ribosome and subsequently captures and translocates the nascent chain. PMID: 18158904

Hide All

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

SecY/SEC61-alpha family

Database Links

KEGG: ecj:JW3262

STRING: 316385.ECDH10B_3475

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Code	CSB-PA314716XA01ENV
Size	US$299
Image	Dot blot analysis of peptides corresponding to the immunogen was probed with secY Antibodyat a dilution of 1:1000. Blocking/Dilution buffer: 4% nonfat dry milk/PBS. Secondary antibody：Goat polyclonal to rabbit IgG at 1/40000 dilution Exposure time: 10s.
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secY Antibody

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