Product Details

Purity

>85% (SDS-PAGE)

Target Names

secY

Uniprot No.

P0AGA2

Alternative Names

secY; prlA; b3300; JW3262; Protein translocase subunit SecY

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.

Gene References into Functions

These data suggest that wild-type YidC residues (G355 and M471) participate in interactions, direct or indirect, with SecY. PMID: 24187090
While translocation of loops 1 and 2 of TatC is strictly dependent on the Sec translocase and the YidC insertase, translocation of loop 3 does not depend on the translocase or insertase. PMID: 23058713
SecY signal peptide is found to specifically bind SecY translocase in a saturable manner and at levels comparable to those that stimulate SecA ATPase activity. PMID: 15476412
provide evidence for the direct interaction between FtsY and the SecY translocon PMID: 15815684
SecY plug domain moves away from the center of the channel toward SecE during polypeptide translocation, and the translocation-enhancing prlA3 mutation and SecG subunit change the properties of channel gating. PMID: 16148946
The data explain how conformational changes in SecA could be directly coupled to the previously proposed opening mechanism of the SecYEG channel. PMID: 16890955
One such translocation complex, SecY/Sec61, is found in every cell, in either the plasma membrane of bacteria and archaea or the endoplasmic reticulum membrane of eukaryotes. PMID: 17077865
the SecY plug contributes to the gating mechanism of the channel by maintaining the structure of the SecYEG complex in a compact closed state PMID: 17092931
normal SecY function is required for the folding of membrane proteins after their insertion into the translocon PMID: 17242069
study used purified E. coli components to address the mechanism of protein translocation; disulfide bridge crosslinking demonstrates that SecA transfers both the signal sequence and the mature region of a secreted substrate into a single SecY molecule PMID: 17418789
The resting cell membrane channel forms a barrier for small molecules, with both the pore ring and the plug required for the seal; channel opening requires movement of the plug. PMID: 17531809
Data suggest that SecY binds to the primary site on the ribosome and subsequently captures and translocates the nascent chain. PMID: 18158904

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

SecY/SEC61-alpha family

Database Links

KEGG: ecj:JW3262

STRING: 316385.ECDH10B_3475

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Recombinant Escherichia coli Protein translocase subunit SecY (secY), partial

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