sep-1 Antibody

Datasheet

Code	CSB-PA271022XA01CXY
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: sep-1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans sep-1 Polyclonal antibody

Uniprot No.

G5ED39

Target Names

sep-1

Alternative Names

Separin homolog sep-1 (EC 3.4.22.49) (Separase) sep-1 Y47G6A.12

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans sep-1 protein (160-215aa)

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Cysteine protease, which plays a central role in homologous chromosome separation during meiosis I and in sister chromatid separation during embryonic mitosis. Promotes chromosome/sister chromatid segregation by cleaving the scc-1 (mitosis) and rec-8 (meiosis) subunits of the cohesin complex at the onset of anaphase. May cleave histone H3-like protein cpar-1 during meiosis I metaphase-anaphase transition. Promotes cortical granule exocytosis after oocyte fertilization during the first meiotic anaphase. Essential for embryonic cytokinesis by regulating rab-11-positive vesicle trafficking at the plasma membrane at the cleavage furrow and midbody. Regulates centriole segregation during spermatocyte meiosis. Required for embryonic anterior-posterior axis formation.

Gene References into Functions

Data indicate that protein phosphatase 5 is an important regulator of separase. PMID: 29246899
These findings suggest that separase cleaves an unknown substrate to promote the exocytosis of RAB-11 vesicles and paves the way for biochemical identification of substrates. PMID: 28820333
single-particle cryo-electron microscopy (cryo-EM) was used to determine a near-atomic-resolution structure of the Caenorhabditis elegans separase-securin complex. PMID: 28263324
Examination of the N-terminal tail sequence of CPAR-1 revealed a putative separase cleavage site and mutation of the signature residues in this site eliminated the cleavage reaction. PMID: 25919583
separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody PMID: 20116245
SEP-1 has two separable yet coordinated functions: to regulate cortical granule exocytosis and to mediate chromosome separation PMID: 17913784

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Subcellular Location

Cytoplasm. Chromosome. Cytoplasmic granule. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody.

Tissue Specificity

Expressed in oocytes. Expressed in male germline. Expressed in spermatocytes but undetectable in spermatids (at protein level).

Database Links

KEGG: cel:CELE_Y47G6A.12

STRING: 6239.Y47G6A.12

UniGene: Cel.17513

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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