Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SGT1B Polyclonal antibody

Uniprot No.

Q9SUT5

Target Names

SGT1B

Alternative Names

SGT1B antibody; EDM1 antibody; ETA3 antibody; RPR1 antibody; At4g11260 antibody; F8L21.50 antibody; Protein SGT1 homolog B antibody; AtSGT1b antibody; Protein ENHANCED DOWNY MILDEW 1 antibody; Protein ENHANCER OF TIR1-1 AUXIN RESISTANCE 3 antibody; Suppressor of G2 allele of SKP1 homolog B antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SGT1B protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in plant innate immunity. Is essential for resistance conferred by multiple R genes recognizing different oomycete pathogen isolates like avirulent H.parasitica (downy mildew). Contributes additively with RAR1 to RPP5-dependent resistance. Not required for RPM1, RPS2, RPS4 and RPS5-mediated resistance. Functions as negative regulator of RPS5 accumulation by assisting its degradation. May be involved in heat shock response by associating with HSC70-1 chaperone. Required for the SCF(TIR1)-mediated degradation of Aux/IAA proteins, but maybe not for SCF(TIR1) assembly or binding to its Aux/IAA substrates. Probably required for SCF-mediated ubiquitination, by coupling HSP90 to SCF complex for ubiquitination of HSP90 client proteins. Required for the coronatine/jasmonic acid-mediated signal transduction pathway.

Gene References into Functions

This study showed that increased temperature promotes rapid accumulation of the TIR1 auxin co-receptor, an effect that is dependent on the molecular chaperone HSP90, and that HSP90 and SGT1 integrate temperature and auxin signaling.[SGT1] PMID: 26728313
SGT1b has a role in disease resistance against Fusarium culmorum. PMID: 19140951
SGT1b antagonizes RAR1 to negatively regulate R protein accumulation before infection, and SGT1b has a RAR1-independent function that regulates programmed cell death during infection [SGT1b] PMID: 15976272
Biochemical reconstitution experiments suggest that RAR1 may function to enhance the SGT1-HSP90 interaction by promoting ternary complex formation. PMID: 18032631
Genetic analysis revealed that ssi4-induced H(2)O(2) accumulation and spontaneous cell death require RAR1, whereas ssi4-mediated stunting is dependent on SGT1b. PMID: 18052881
Data show that intact C-terminal SGT1-specific (SGS) domain that is required for all known SGT1b functions in immunity and development is needed for HSC70 interaction and for the nuclear accumulation of SGT1b. PMID: 18065690

Hide All

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

SGT1 family

Database Links

KEGG: ath:AT4G11260

STRING: 3702.AT4G11260.1

UniGene: At.23379

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA865990XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

SGT1B Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background