Recombinant Arabidopsis thaliana Protein SGT1 homolog B (SGT1B)

Code CSB-YP865990DOA
MSDS
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Source Yeast
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Code CSB-EP865990DOA
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Source E.coli
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Code CSB-EP865990DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865990DOA
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Source Baculovirus
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Code CSB-MP865990DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SGT1B
Uniprot No.
Alternative Names
SGT1B; EDM1; ETA3; RPR1; At4g11260; F8L21.50; Protein SGT1 homolog B; AtSGT1b; Protein ENHANCED DOWNY MILDEW 1; Protein ENHANCER OF TIR1-1 AUXIN RESISTANCE 3; Suppressor of G2 allele of SKP1 homolog B
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-358
Target Protein Sequence
MAKELAEKAK EAFLDDDFDV AVDLYSKAID LDPNCAAFFA DRAQANIKID NFTEAVVDAN KAIELEPTLA KAYLRKGTAC MKLEEYSTAK AALEKGASVA PNEPKFKKMI DECDLRIAEE EKDLVQPMPP SLPSSSTTPL ATEADAPPVP IPAAPAKPMF RHEFYQKPEE AVVTIFAKKV PKENVTVEFG EQILSVVIDV AGEEAYHLQP RLFGKIIPEK CRFEVLSTKV EIRLAKAEII TWASLEYGKG QSVLPKPNVS SALSQRPVYP SSKPAKDWDK LEAEVKKQEK DEKLDGDAAM NKFFSDIYSS ADEDMRRAMN KSFAESNGTV LSTNWKEVGT KKVESTPPDG MELKKWEY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in plant innate immunity. Is essential for resistance conferred by multiple R genes recognizing different oomycete pathogen isolates like avirulent H.parasitica (downy mildew). Contributes additively with RAR1 to RPP5-dependent resistance. Not required for RPM1, RPS2, RPS4 and RPS5-mediated resistance. Functions as negative regulator of RPS5 accumulation by assisting its degradation. May be involved in heat shock response by associating with HSC70-1 chaperone. Required for the SCF(TIR1)-mediated degradation of Aux/IAA proteins, but maybe not for SCF(TIR1) assembly or binding to its Aux/IAA substrates. Probably required for SCF-mediated ubiquitination, by coupling HSP90 to SCF complex for ubiquitination of HSP90 client proteins. Required for the coronatine/jasmonic acid-mediated signal transduction pathway.
Gene References into Functions
  1. This study showed that increased temperature promotes rapid accumulation of the TIR1 auxin co-receptor, an effect that is dependent on the molecular chaperone HSP90, and that HSP90 and SGT1 integrate temperature and auxin signaling.[SGT1] PMID: 26728313
  2. SGT1b has a role in disease resistance against Fusarium culmorum. PMID: 19140951
  3. SGT1b antagonizes RAR1 to negatively regulate R protein accumulation before infection, and SGT1b has a RAR1-independent function that regulates programmed cell death during infection [SGT1b] PMID: 15976272
  4. Biochemical reconstitution experiments suggest that RAR1 may function to enhance the SGT1-HSP90 interaction by promoting ternary complex formation. PMID: 18032631
  5. Genetic analysis revealed that ssi4-induced H(2)O(2) accumulation and spontaneous cell death require RAR1, whereas ssi4-mediated stunting is dependent on SGT1b. PMID: 18052881
  6. Data show that intact C-terminal SGT1-specific (SGS) domain that is required for all known SGT1b functions in immunity and development is needed for HSC70 interaction and for the nuclear accumulation of SGT1b. PMID: 18065690

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
SGT1 family
Database Links

KEGG: ath:AT4G11260

STRING: 3702.AT4G11260.1

UniGene: At.23379

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