EndoA Antibody

Code CSB-PA844691XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) EndoA Polyclonal antibody
Uniprot No.
Target Names
EndoA
Alternative Names
EndoA antibody; endo antibody; CG14296 antibody; Endophilin-A antibody; SH3 domain-containing GRB2-like protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) EndoA protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.
Gene References into Functions
  1. EndophilinA-induced macroautophagy is activated when the kinase LRRK2 phosphorylates the EndophilinA-BAR domain and is blocked in animals where EndophilinA cannot be phosphorylated. Functionally, deregulation of the EndophilinA phosphorylation state accelerates activity-induced neurodegeneration. PMID: 27720484
  2. analysis of endophilin-A N-BAR domain mutations performed in living flies PMID: 20209138
  3. endophilin A is essential for synaptic vesicle endocytosis, being required from the onset of budding until fission. PMID: 11927550
  4. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo PMID: 11955450
  5. At fly photoreceptor synapses Endophilin is localized to synaptic vesicles at sites of endocytosis that are glial invaginations called capitate projections; when the photoreceptor synapses lack Endophilin they are impaired in neurotransmitter release PMID: 14627659

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Protein Families
Endophilin family
Tissue Specificity
Expression is abundant in central nervous system (brain lobes and the ventral nerve cord) starting at embryonic stage 13 through to third instar larvae. Also expressed in photoreceptors in the eye imaginal disks and the Bolwig organs of the peripheral ner
Database Links

KEGG: dme:Dmel_CG14296

STRING: 7227.FBpp0083112

UniGene: Dm.3503

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