Recombinant Drosophila melanogaster Endophilin-A (endoA)

Code CSB-YP844691DLU
MSDS
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Source Yeast
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Code CSB-EP844691DLU
MSDS
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Source E.coli
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Code CSB-EP844691DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844691DLU
MSDS
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Source Baculovirus
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Code CSB-MP844691DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EndoA
Uniprot No.
Alternative Names
EndoA; endo; CG14296; Endophilin-A; SH3 domain-containing GRB2-like protein
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-369
Target Protein Sequence
MAFAGLKKQI NKANQYMTEK MGGAEGTKLD MDFMEMERKT DVTVELVEEL QLKTKEFLQP NPTARAKMAA VKGISKLSGQ AKSNTYPQPE GLLAECMLTY GKKLGEDNSV FAQALVEFGE ALKQMADVKY SLDDNIKQNF LEPLHHMQTK DLKEVMHHRK KLQGRRLDFD CKRRRQAKDD EIRGAEDKFG ESLQLAQVGM FNLLENDTEH VSQLVTFAEA LYDFHSQCAD VLRGLQETLQ EKRSEAESRP RNEFVPKTLL DLNLDGGGGG LNEDGTPSHI SSSASPLPSP MRSPAKSMAV TPQRQQQPCC QALYDFEPEN PGELAFKEND IITLLNRVDD NWFEGAVNGR TGYFPQSYVQ VQVPLPNGN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.
Gene References into Functions
  1. EndophilinA-induced macroautophagy is activated when the kinase LRRK2 phosphorylates the EndophilinA-BAR domain and is blocked in animals where EndophilinA cannot be phosphorylated. Functionally, deregulation of the EndophilinA phosphorylation state accelerates activity-induced neurodegeneration. PMID: 27720484
  2. analysis of endophilin-A N-BAR domain mutations performed in living flies PMID: 20209138
  3. endophilin A is essential for synaptic vesicle endocytosis, being required from the onset of budding until fission. PMID: 11927550
  4. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo PMID: 11955450
  5. At fly photoreceptor synapses Endophilin is localized to synaptic vesicles at sites of endocytosis that are glial invaginations called capitate projections; when the photoreceptor synapses lack Endophilin they are impaired in neurotransmitter release PMID: 14627659

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Protein Families
Endophilin family
Tissue Specificity
Expression is abundant in central nervous system (brain lobes and the ventral nerve cord) starting at embryonic stage 13 through to third instar larvae. Also expressed in photoreceptors in the eye imaginal disks and the Bolwig organs of the peripheral ner
Database Links

KEGG: dme:Dmel_CG14296

STRING: 7227.FBpp0083112

UniGene: Dm.3503

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