SIN3 Antibody

Code CSB-PA332845XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIN3 Polyclonal antibody
Uniprot No.
Target Names
SIN3
Alternative Names
SIN3 antibody; CPE1 antibody; GAM2 antibody; RPD1 antibody; SDI1 antibody; SDS16 antibody; UME4 antibody; YOL004W antibody; Transcriptional regulatory protein SIN3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIN3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalytic component of the RPD3 histone deacetylase complexes RPD3C(L) and RPD3C(S) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. SIN3 has also a RPD3 independent function required for normal longevity.
Gene References into Functions
  1. Based on findings, authors conclude that the Tor and PpSin3-PpRpd3 pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation. PMID: 24838945
  2. These results show that the sin3 mutation decreases both spontaneous and UV-induced levels of levels. PMID: 25711007
  3. investigated the regulatory contribution of subunits of Sin3 complexes and identified Pho23 as important for inositol and choline-dependent gene repression PMID: 22543816
  4. PAH2 and PAH3 od sin3 are required for mitotic early meiotic gene repression. PMID: 20971827
  5. Mutational analysis of the Opi1-Sin3 interaction domain (OSID) revealed that hydrophobic OSID residues L56, V59 and V67 of Opi1 are indispensable for gene repression PMID: 21104417
  6. The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase. PMID: 15141165
  7. The Sin3/HDAC complex is involved in an array of biological and cellular processes, including cell cycle progression, genomic stability, embryonic development, and homeostasis. Review. PMID: 19505602
  8. Sin3/Rpd3 histone deacetylase complex helps to prevent premature activation of the S phase in daughter cells. PMID: 19523118

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YOL004W

STRING: 4932.YOL004W

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