SIR1 Antibody

Code CSB-PA325134XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIR1 Polyclonal antibody
Uniprot No.
Target Names
SIR1
Alternative Names
SIR1 antibody; YKR101W antibody; Regulatory protein SIR1 antibody; Heterochromatin protein SIR1 antibody; Silent information regulator 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIR1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the establishment, but not the maintenance, of heterochromatic silencing at the cryptic mating-type loci HMR and HML. Is recruited by interacting with the ORC1 subunit of the origin recognition complex (ORC), which binds to HML-I or HMR-E silencers, DNA elements that direct the formation of silent chromatin at the mating-type loci. Establishes transcriptional silencing by recruiting the three other SIR proteins, SIR2, SIR3, and SIR4, that function directly in silenced chromatin and establish repression. Also found in centromeric chromatin. Binds to and helps retain CAC1, a subunit of chromatin assembly factor I (CAF-I) at centromeric loci independent on the other SIR proteins.
Gene References into Functions
  1. data provide the most complete picture to date of how the earliest SIR1-containing SIR silencing complex functioned and the evolutionary trajectories it may have followed PMID: 26811328
  2. SIR1 plays role in the origin recognition complex dependent silencing for origin firing at silent mating type loci PMID: 16479013
  3. The role for Ku in silencing both HMR and HML was masked by SIR1. PMID: 18716325
  4. The functional duality might contribute to Sir1's selectivity for silencer-bound origin recognition complex in vivo. PMID: 19029247
Subcellular Location
Nucleus. Chromosome, centromere. Note=Associated primarily with the HMR-E silencer at the HMR locus.
Database Links

KEGG: sce:YKR101W

STRING: 4932.YKR101W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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