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Recombinant Saccharomyces cerevisiae Regulatory protein SIR1 (SIR1), partial

Recombinant Saccharomyces cerevisiae Regulatory protein SIR1 (SIR1), partial

Code	CSB-YP325134SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP325134SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP325134SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP325134SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP325134SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SIR1

Uniprot No.

P21691

Alternative Names

SIR1; YKR101W; Regulatory protein SIR1; Heterochromatin protein SIR1; Silent information regulator 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in the establishment, but not the maintenance, of heterochromatic silencing at the cryptic mating-type loci HMR and HML. Is recruited by interacting with the ORC1 subunit of the origin recognition complex (ORC), which binds to HML-I or HMR-E silencers, DNA elements that direct the formation of silent chromatin at the mating-type loci. Establishes transcriptional silencing by recruiting the three other SIR proteins, SIR2, SIR3, and SIR4, that function directly in silenced chromatin and establish repression. Also found in centromeric chromatin. Binds to and helps retain CAC1, a subunit of chromatin assembly factor I (CAF-I) at centromeric loci independent on the other SIR proteins.

Gene References into Functions

data provide the most complete picture to date of how the earliest SIR1-containing SIR silencing complex functioned and the evolutionary trajectories it may have followed PMID: 26811328
SIR1 plays role in the origin recognition complex dependent silencing for origin firing at silent mating type loci PMID: 16479013
The role for Ku in silencing both HMR and HML was masked by SIR1. PMID: 18716325
The functional duality might contribute to Sir1's selectivity for silencer-bound origin recognition complex in vivo. PMID: 19029247

Subcellular Location

Nucleus. Chromosome, centromere. Note=Associated primarily with the HMR-E silencer at the HMR locus.

Database Links

KEGG: sce:YKR101W

STRING: 4932.YKR101W

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