slyD Antibody

Code CSB-PA359316XA01EOD
Size US$299
Image
  • Dot blot analysis of peptides corresponding to the immunogen was probed with slyD Antibodyat a dilution of 1:1000. Blocking/Dilution buffer: 4% nonfat dry milk/PBS. Secondary antibody:Goat polyclonal to rabbit IgG at 1/40000 dilution Exposure time: 30s.
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 slyD Polyclonal antibody
Uniprot No.
Target Names
slyD
Alternative Names
FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (PPIase) (EC 5.2.1.8) (Metallochaperone SlyD) slyD Z4707 ECs4200
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7
Immunogen
Synthesized peptide derived from internal of Escherichia coli O157:H7 slyD protein (73-86aa )
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Folding helper with both chaperone and peptidyl-prolyl cis-trans isomerase (PPIase) activities. Chaperone activity prevents aggregation of unfolded or partially folded proteins and promotes their correct folding. PPIases catalyze the cis-trans isomerization of Xaa-Pro bonds of peptides, which accelerates slow steps of protein folding and thus shortens the lifetime of intermediates. Both strategies lower the concentration of intermediates and increase the productivity and yield of the folding reaction. SlyD could be involved in Tat-dependent translocation, by binding to the Tat-type signal of folded proteins.; Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity.
Subcellular Location
Cytoplasm.
Protein Families
FKBP-type PPIase family
Database Links

KEGG: ece:Z4707

STRING: 155864.Z4707

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