Product Details

Full Product Name

Rabbit anti-Xenopus laevis (African clawed frog) sox3-a Polyclonal antibody

Uniprot No.

P55863

Target Names

sox3-a

Alternative Names

sox3-a antibody; sox3 antibody; Transcription factor Sox-3-A antibody; xSox3 antibody; xSox-B1 antibody

Raised in

Rabbit

Species Reactivity

Xenopus laevis (African clawed frog)

Immunogen

Recombinant Xenopus laevis (African clawed frog) sox3-a protein

Immunogen Species

Xenopus laevis (African clawed frog)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription factor with sequence-specific DNA binding activity. Binds to the consensus sequence 5'-[AT][AT]CAA[AT]G-3', showing a preference for 5'-AACAAT-3' and 5'-AACAAAG-3'. Inhibits beta-catenin-mediated dorsal axis specification by binding to sites within the promoter of the beta-catenin-regulated gene nodal5. Acts maternally as a transcriptional repressor of nodal5 and nodal6 to restrict their expression to the vegetal hemisphere of early embryos and thus establish germ layer formation. Acts at multiple points to inhibit nodal signaling, repressing the expression of the other mesoderm-inducing nodal genes nodal, nodal2 and nodal4, and also acting downstream to induce expression of genes including trim33/ectodermin, ema and coco, whose products repress nodal signaling.

Gene References into Functions

XSeb4R protein bound specifically to the 3'UTR of Sox3 mRNA. XSeb4R gain-of-function in ectodermal explants revealed increased stability of the maternal Sox3 transcripts, associated with a robust Sox3 protein production. PMID: 22261149
knockdown of Oct91 inhibits neural induction driven by either Sox2 or Sox3. PMID: 21147085
Animal pole localized Sox3 acts to inhibit Nodal (Xnr5 and Xnr6) expression, and induces the expression of genes (Ectodermin, Xema, and Coco) whose products repress Nodal signaling. PMID: 17608734
Results demonstrate that both sox3 and sox2 are induced in response to BMP antagonism, but by distinct mechanisms and that the activation of both genes is independent of FGF signaling; however, both require FGF for the maintenance of their expression. PMID: 18031719

Subcellular Location

Nucleus. Cytoplasm. Note=Primarily cytoplasmic in early embryos. Nuclear localization becomes more pronounced as development proceeds.

Tissue Specificity

Expression is restricted to the animal hemisphere of the uncleaved egg and early cleavage embryos. In late blastula and gastrula stages, expressed in the dorsal animal and marginal zone. In neurula stages, expressed in the developing central nervous syste

Database Links

KEGG: xla:399335

UniGene: Xl.22

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sox3-a Antibody

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