Recombinant Xenopus laevis Transcription factor Sox-3-A (sox3-a)

Code CSB-YP345910XBE
MSDS
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Source Yeast
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Code CSB-EP345910XBE
MSDS
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Source E.coli
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Code CSB-EP345910XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345910XBE
MSDS
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Source Baculovirus
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Code CSB-MP345910XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sox3-a
Uniprot No.
Alternative Names
sox3-a; sox3; Transcription factor Sox-3-A; xSox3; xSox-B1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-309
Target Protein Sequence
MYSMLDTDIK SPVQQSNAPI GGPATPGGKG NASTLDQDRV KRPMNAFMVW SRGQRRKMAQ ENPKMHNSEI SKRLGADWKL LSDSDKRPFI DEAKRLRAVH MKDYPDYKYR PRRKTKTLLK KDKYSLPGNL LAPGVSPVAS SVGVGQRIDT YAHMNGWTNG AYSLMQDQLG YSQHPAMNSP QMQQIQHRYD MSGLQYNPMM TSAQNAYMNA AASTYSMSPA YNQQSSTVMS LASMGSVVKS EPSSPPPAIT SHTQRACLGD LRDMISMYLP PGGDASDPSL QNSRLHSVHQ HYQSAAGPGV NGTVPLTHI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor with sequence-specific DNA binding activity. Binds to the consensus sequence 5'-[AT][AT]CAA[AT]G-3', showing a preference for 5'-AACAAT-3' and 5'-AACAAAG-3'. Inhibits beta-catenin-mediated dorsal axis specification by binding to sites within the promoter of the beta-catenin-regulated gene nodal5. Acts maternally as a transcriptional repressor of nodal5 and nodal6 to restrict their expression to the vegetal hemisphere of early embryos and thus establish germ layer formation. Acts at multiple points to inhibit nodal signaling, repressing the expression of the other mesoderm-inducing nodal genes nodal, nodal2 and nodal4, and also acting downstream to induce expression of genes including trim33/ectodermin, ema and coco, whose products repress nodal signaling.
Gene References into Functions
  1. XSeb4R protein bound specifically to the 3'UTR of Sox3 mRNA. XSeb4R gain-of-function in ectodermal explants revealed increased stability of the maternal Sox3 transcripts, associated with a robust Sox3 protein production. PMID: 22261149
  2. knockdown of Oct91 inhibits neural induction driven by either Sox2 or Sox3. PMID: 21147085
  3. Animal pole localized Sox3 acts to inhibit Nodal (Xnr5 and Xnr6) expression, and induces the expression of genes (Ectodermin, Xema, and Coco) whose products repress Nodal signaling. PMID: 17608734
  4. Results demonstrate that both sox3 and sox2 are induced in response to BMP antagonism, but by distinct mechanisms and that the activation of both genes is independent of FGF signaling; however, both require FGF for the maintenance of their expression. PMID: 18031719
Subcellular Location
Nucleus. Cytoplasm. Note=Primarily cytoplasmic in early embryos. Nuclear localization becomes more pronounced as development proceeds.
Tissue Specificity
Expression is restricted to the animal hemisphere of the uncleaved egg and early cleavage embryos. In late blastula and gastrula stages, expressed in the dorsal animal and marginal zone. In neurula stages, expressed in the developing central nervous syste
Database Links

KEGG: xla:399335

UniGene: Xl.22

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