spoIISA Antibody

Datasheet

Code	CSB-PA520274XA01BRJ
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Target Background

Product Details

Full Product Name

Rabbit anti-Bacillus subtilis (strain 168) spoIISA Polyclonal antibody

Uniprot No.

O34853

Target Names

spoIISA

Alternative Names

spoIISA; ykaC; BSU12830; Stage II sporulation protein SA; Killer protein SpoIISA; Toxin SpoIISA

Raised in

Rabbit

Species Reactivity

Bacillus subtilis (strain 168)

Immunogen

Recombinant Bacillus subtilis (strain 168) spoIISA protein

Immunogen Species

Bacillus subtilis (strain 168)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Toxic component of a type II toxin-antitoxin (TA) system. Its toxic activity is neutralized by cognate antitoxin SpoIISB. Expression in the absence of SpoIISB permits sporulation to stage II, when plasmolysis zones and holes in the peptidoglycan layer are observed, resulting in cell death. Lethal when synthesized during vegetative growth in the absence of SpoIISB. In E.coli both the membrane bound and soluble domain are required in cis for toxin activity.

Gene References into Functions

Using truncated SpoIISA constructs, authors show that the entire transmembrane domain is required for its toxicity. PMID: 25039482
SpoIISA levels increase in stationary phase and localized to cytoplasmic membrane. PMID: 20863891

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

SpoIISA toxin family

Database Links

KEGG: bsu:BSU12830

STRING: 224308.Bsubs1_010100007111

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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spoIISA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background