Recombinant Bacillus subtilis Stage II sporulation protein SA (spoIISA)

Code CSB-CF520274BRJ
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
spoIISA
Uniprot No.
Alternative Names
spoIISA; ykaC; BSU12830; Stage II sporulation protein SA; Killer protein SpoIISA; Toxin SpoIISA
Species
Bacillus subtilis (strain 168)
Expression Region
1-248
Target Protein Sequence
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSL FTHWERYLIVAVSFALIDAFIFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTY QYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASLCHYSTQADKDRLTEHMDDPA DVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL PIEEEGEG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Toxic component of a type II toxin-antitoxin (TA) system. Its toxic activity is neutralized by cognate antitoxin SpoIISB. Expression in the absence of SpoIISB permits sporulation to stage II, when plasmolysis zones and holes in the peptidoglycan layer are observed, resulting in cell death. Lethal when synthesized during vegetative growth in the absence of SpoIISB. In E.coli both the membrane bound and soluble domain are required in cis for toxin activity.
Gene References into Functions
  1. Using truncated SpoIISA constructs, authors show that the entire transmembrane domain is required for its toxicity. PMID: 25039482
  2. SpoIISA levels increase in stationary phase and localized to cytoplasmic membrane. PMID: 20863891
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
SpoIISA toxin family
Database Links
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