SPA1 Antibody

Code CSB-PA879885XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPA1 Polyclonal antibody
Uniprot No.
Target Names
SPA1
Alternative Names
SPA1 antibody; At2g46340/At2g46350 antibody; F11C10.3/F11C10.4 antibody; Protein SUPPRESSOR OF PHYA-105 1 antibody; EC 2.7.-.- antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SPA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Controls normal photoperiodic flowering and regulates circadian rhythms. Required for suppression of photomorphogenesis in dark-grown seedlings and for normal elongation growth of adult plants. Integral component of the COP1/SPA E3 ubiquitin-protein ligase complex. Involved in HY5, HFR1, LAF1 and CO degradation.
Gene References into Functions
  1. The WD-repeat domain of SPA1 is necessary for the interaction between SPA1 and COL12. PMID: 29187570
  2. The N-terminal kinase-like domain is primarily responsible for the respective light-responsiveness of SPA1 and SPA2. PMID: 27444995
  3. these results provide insights into SPA1- and MYC2-mediated transcriptional regulation of the Z- and G-box containing promoters in light signaling pathways. PMID: 23646119
  4. CRY2 directly interacts with SPA1 in nuclei PMID: 22739826
  5. SPA1 Is Required for the CRY2-Mediated Photoperiodic Regulation of Floral Initiation. PMID: 21514160
  6. demonstrated the genetic and molecular relationships of MYC2 and SPA1 in light and JA (jasmonic acid) signaling pathways. Here, we have further shown the genetic interactions between these two proteins in flowering time and lateral root development PMID: 21512327
  7. CRY1 interacts physically with SPA1 in a blue-light-dependent manner. PMID: 21511872
  8. SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time. PMID: 21447551
  9. SPA1 and SPA2 proteins are rapidly de-stabilized upon light exposure. PMID: 21235648
  10. MYC2 and SPA1 act redundantly to suppress photomorphogenic growth in the dark. PMID: 20864543
  11. Results show that SPA1 and DET1 act together to control photomorphogenesis throughout plant development. PMID: 20041285
  12. Repression of light signaling by Arabidopsis SPA1 likely involves post-translational regulation of HFR1 protein accumulation. PMID: 15960622
  13. SPA1 regulates circadian rhythms and flowering time in plants. PMID: 16709190
  14. SPA1 protein can self-associate or interact with each other, forming stable heterogeneous group of SPA-COP1 complexes regardless of light conditions but exhibit distinct expression profiles in different tissues and light conditions. PMID: 18812498

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Subcellular Location
Nucleus speckle. Nucleus. Nucleus, PML body.
Database Links

KEGG: ath:AT2G46340

STRING: 3702.AT2G46340.1

UniGene: At.11156

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