SPO11-1 Antibody

Datasheet

Code	CSB-PA868126XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPO11-1 Polyclonal antibody

Uniprot No.

Q9M4A2

Target Names

SPO11-1

Alternative Names

SPO11-1 antibody; At3g13170 antibody; MJG19.19 antibody; Meiotic recombination protein SPO11-1 antibody; AtSPO11-1 antibody; EC 5.6.2.2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SPO11-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles.

Gene References into Functions

As transposon mobility in plant genomes is restricted by DNA methylation, we used the met1 DNA methyltransferase mutant to investigate the role of heterochromatin in SPO11-1-oligo distributions. Epigenetic activation of meiotic DSBs in proximity to centromeres and transposons occurred in met1 mutants, coincident with reduced nucleosome occupancy, gain of transcription, and H3K4me3 PMID: 29530928
Results identified a group of spo11-1 mutants with defects in meiosis that specifically correlate with the defects in DNA binding at an identified DNA binding surface on SPO11-1. PMID: 20423461
AtSPO11-1 and AtSPO11-2 genetically interact and function in meiotic recombination. [AtSPO11-1] PMID: 17018031
Data show that the catalytically active tyrosine residues of SPO11-1 are required for meiotic double-strand break induction in Arabidopsis. PMID: 17965269

Subcellular Location

Nucleus.

Protein Families

TOP6A family

Tissue Specificity

Expressed in shoots, young seedlings, flowers and reproductive tissues. Not found in roots or rosette leaves.

Database Links

KEGG: ath:AT3G13170

STRING: 3702.AT3G13170.1

UniGene: At.48652

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SPO11-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background