Recombinant Arabidopsis thaliana Meiotic recombination protein SPO11-1 (SPO11-1)

Code CSB-YP868126DOA
MSDS
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Source Yeast
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Code CSB-EP868126DOA
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Source E.coli
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Code CSB-EP868126DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868126DOA
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Source Baculovirus
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Code CSB-MP868126DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPO11-1
Uniprot No.
Alternative Names
SPO11-1; At3g13170; MJG19.19; Meiotic recombination protein SPO11-1; AtSPO11-1; EC 5.6.2.2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-362
Target Protein Sequence
MEGKFAISES TNLLQRIKDF TQSVVVDLAE GRSPKISINQ FRNYCMNPEA DCLCSSDKPK GQEIFTLKKE PQTYRIDMLL RVLLIVQQLL QENRHASKRD IYYMHPSAFK AQSIVDRAIG DICILFQCSR YNLNVVSVGN GLVMGWLKFR EAGRKFDCLN SLNTAYPVPV LVEEVEDIVS LAEYILVVEK ETVFQRLAND MFCKTNRCIV ITGRGYPDVS TRRFLRLLME KLHLPVHCLV DCDPYGFEIL ATYRFGSMQM AYDIESLRAP DMKWLGAFPS DSEVYSVPKQ CLLPLTEEDK KRTEAMLLRC YLKREMPQWR LELETMLKRG VKFEIEALSV HSLSFLSEVY IPSKIRREVS SP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles.
Gene References into Functions
  1. As transposon mobility in plant genomes is restricted by DNA methylation, we used the met1 DNA methyltransferase mutant to investigate the role of heterochromatin in SPO11-1-oligo distributions. Epigenetic activation of meiotic DSBs in proximity to centromeres and transposons occurred in met1 mutants, coincident with reduced nucleosome occupancy, gain of transcription, and H3K4me3 PMID: 29530928
  2. Results identified a group of spo11-1 mutants with defects in meiosis that specifically correlate with the defects in DNA binding at an identified DNA binding surface on SPO11-1. PMID: 20423461
  3. AtSPO11-1 and AtSPO11-2 genetically interact and function in meiotic recombination. [AtSPO11-1] PMID: 17018031
  4. Data show that the catalytically active tyrosine residues of SPO11-1 are required for meiotic double-strand break induction in Arabidopsis. PMID: 17965269
Subcellular Location
Nucleus.
Protein Families
TOP6A family
Tissue Specificity
Expressed in shoots, young seedlings, flowers and reproductive tissues. Not found in roots or rosette leaves.
Database Links

KEGG: ath:AT3G13170

STRING: 3702.AT3G13170.1

UniGene: At.48652

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