SPT Antibody

Code CSB-PA867014XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPT Polyclonal antibody
Uniprot No.
Target Names
SPT
Alternative Names
SPT antibody; BHLH24 antibody; EN99 antibody; At4g36930 antibody; AP22.25 antibody; C7A10.430 antibody; Transcription factor SPATULA antibody; Basic helix-loop-helix protein 24 antibody; AtbHLH24 antibody; bHLH 24 antibody; Transcription factor EN 99 antibody; bHLH transcription factor bHLH024 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SPT protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that plays a role in floral organogenesis. Promotes the growth of carpel margins and of pollen tract tissues derived from them.
Gene References into Functions
  1. SPT is a growth repressor that acts to limit the size of meristems in response to environmental signals, perhaps by regulating auxin transport. PMID: 23470719
  2. SPT has opposite roles in setting seed dormancy in Landsberg erecta and Columbia Arabidopsis ecotypes PMID: 23754415
  3. The data suggests that SPT plays a role in control of root growth, similar to its roles in above ground tissues. PMID: 23280064
  4. SPATULA (SPT) regulates gynoecium development by activating genes involved in shade avoidance. PMID: 22851763
  5. Over-expression of ALCATRAZ can partially compensate for a loss of SPT function, and over-expression of SPT can fully compensate for loss of ALCATRAZ function. PMID: 21801252
  6. SPT integrates time of day and temperature signaling to control vegetative growth rate. PMID: 20705468
  7. SPT is necessary in limiting leaf size without dramatically affecting leaf shape by restricting the size of the meristematic region in leaf primordia independently of the AN3-AtGRF5 pathway. PMID: 20040585
  8. SPATULA is a light-stable repressor of seed germination and mediates the germination response to temperature. PMID: 16303558
  9. conserved acidic domain is essential for carpel function, supported by conserved amphipathic helix PMID: 18315540

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in lateral root caps, young leaves, stipules, maturing pith cells of the stem, differentiating vascular cells, shoot apical meristems and flowers.
Database Links

KEGG: ath:AT4G36930

STRING: 3702.AT4G36930.1

UniGene: At.4639

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