Recombinant Arabidopsis thaliana Transcription factor SPATULA (SPT)

Code CSB-YP867014DOA
MSDS
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Source Yeast
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Code CSB-EP867014DOA
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Source E.coli
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Code CSB-EP867014DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867014DOA
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Source Baculovirus
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Code CSB-MP867014DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPT
Uniprot No.
Alternative Names
SPT; BHLH24; EN99; At4g36930; AP22.25; C7A10.430; Transcription factor SPATULA; Basic helix-loop-helix protein 24; AtbHLH24; bHLH 24; Transcription factor EN 99; bHLH transcription factor bHLH024
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-373
Target Protein Sequence
MISQREEREE KKQRVMGDKK LISSSSSSSV YDTRINHHLH HPPSSSDEIS QFLRHIFDRS SPLPSYYSPA TTTTTASLIG VHGSGDPHAD NSRSLVSHHP PSDSVLMSKR VGDFSEVLIG GGSGSAAACF GFSGGGNNNN VQGNSSGTRV SSSSVGASGN ETDEYDCESE EGGEAVVDEA PSSKSGPSSR SSSKRCRAAE VHNLSEKRRR SRINEKMKAL QSLIPNSNKT DKASMLDEAI EYLKQLQLQV QMLTMRNGIN LHPLCLPGTT LHPLQLSQIR PPEATNDPLL NHTNQFASTS NAPEMINTVA SSYALEPSIR SHFGPFPLLT SPVEMSREGG LTHPRLNIGH SNANITGEQA LFDGQPDLKD RIT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that plays a role in floral organogenesis. Promotes the growth of carpel margins and of pollen tract tissues derived from them.
Gene References into Functions
  1. SPT is a growth repressor that acts to limit the size of meristems in response to environmental signals, perhaps by regulating auxin transport. PMID: 23470719
  2. SPT has opposite roles in setting seed dormancy in Landsberg erecta and Columbia Arabidopsis ecotypes PMID: 23754415
  3. The data suggests that SPT plays a role in control of root growth, similar to its roles in above ground tissues. PMID: 23280064
  4. SPATULA (SPT) regulates gynoecium development by activating genes involved in shade avoidance. PMID: 22851763
  5. Over-expression of ALCATRAZ can partially compensate for a loss of SPT function, and over-expression of SPT can fully compensate for loss of ALCATRAZ function. PMID: 21801252
  6. SPT integrates time of day and temperature signaling to control vegetative growth rate. PMID: 20705468
  7. SPT is necessary in limiting leaf size without dramatically affecting leaf shape by restricting the size of the meristematic region in leaf primordia independently of the AN3-AtGRF5 pathway. PMID: 20040585
  8. SPATULA is a light-stable repressor of seed germination and mediates the germination response to temperature. PMID: 16303558
  9. conserved acidic domain is essential for carpel function, supported by conserved amphipathic helix PMID: 18315540

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in lateral root caps, young leaves, stipules, maturing pith cells of the stem, differentiating vascular cells, shoot apical meristems and flowers.
Database Links

KEGG: ath:AT4G36930

STRING: 3702.AT4G36930.1

UniGene: At.4639

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