SPY Antibody

^{Rare Species}

Datasheet

Code	CSB-PA300608XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: SPY antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26.8 kDa Observed band size: 33 kDa
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Product Details

Uniprot No.

P77754

Target Names

SPY

Alternative Names

Periplasmic chaperone Spy (Spheroplast protein Y) spy b1743 JW1732

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) SPY protein (52-147aa)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface. Substrate protein folds while it is bound to chaperone. Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity. Overexpression enhances the stability of otherwise unstable periplasmic proteins.

Gene References into Functions

Visualizing of Spy chaperone-assisted Im7 protein folding has been reported. PMID: 27239796
Experimental results on Spy-Im7 interactions were used to construct a model for how this chaperone interacts with its clients. It appears that Spy binds to and releases client proteins in a regulated fashion that uses client protein folding as its own cue for release. PMID: 27293188
Spy rapidly associates with its substrate, immunity protein 7 (Im7), thereby eliminating Im7's potential for aggregation. PMID: 26619265
Establish a good relationship between the degree of instability and the improvement chaperone activity of spy variants. PMID: 24497545
Report of the crystal structure of Escherichia coli spheroplast protein y (EcSpy). PMID: 20799348
The induction of spy gene transcription by external zinc may include multiple steps such as through sensing the zinc-induced envelope disorder by BaeSR. PMID: 17884222

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Subcellular Location

Periplasm.

Protein Families

CpxP/Spy family

Database Links

KEGG: ecj:JW1732

STRING: 316385.ECDH10B_1881

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SPY Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background