KN Antibody

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Code	CSB-PA670356XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) KN Polyclonal antibody

Uniprot No.

Q42374

Target Names

Alternative Names

KN antibody; SYP111 antibody; At1g08560 antibody; F22O13.4 antibody; Syntaxin-related protein KNOLLE antibody; Syntaxin-111 antibody; AtSYP111 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana KN protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in cytokinesis. Acts as a cell plate-specific syntaxin, required for the fusion of vesicles at the plane of cell division.

Gene References into Functions

Immunolocalization of the cytokinesis-specific syntaxin KNOLLE (KN) in ap1m2 showed that KN was mislocalized and aggregated around the division plane. PMID: 23543752
Two distinct types of KNOLLE complexes appear to jointly mediate membrane fusion in Arabidopsis cytokinesis. PMID: 23515225
KEULE interacted with an open conformation of KNOLLE that complemented both knolle and keule mutants. This interaction occurred at the cell division plane and required the KNOLLE linker sequence between helix Hc and SNARE domain. PMID: 22595672
KNOLLE regions are necessary for syntaxin function in cytokinesis. PMID: 21838778
Restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis. PMID: 19959995

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Subcellular Location

Membrane; Single-pass type IV membrane protein.

Protein Families

Syntaxin family

Tissue Specificity

Abundant in flowers and developing siliques. A low level expression is seen in the seedlings, roots, and leaves.

Database Links

KEGG: ath:AT1G08560

STRING: 3702.AT1G08560.1

UniGene: At.10802

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KN Antibody

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