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Recombinant Arabidopsis thaliana Syntaxin-related protein KNOLLE (KN), partial

Code CSB-YP670356DOA
MSDS
MSDS Datasheet
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Source Yeast
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Code CSB-EP670356DOA
MSDS
MSDS Datasheet
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Source E.coli
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Code CSB-EP670356DOA-B
MSDS
MSDS Datasheet
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP670356DOA
MSDS
MSDS Datasheet
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Source Baculovirus
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Code CSB-MP670356DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KN
Uniprot No.
Q42374
Alternative Names
KN; SYP111; At1g08560; F22O13.4; Syntaxin-related protein KNOLLE; Syntaxin-111; AtSYP111
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Protein FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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KN Antibodies

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Target Background

Function
Involved in cytokinesis. Acts as a cell plate-specific syntaxin, required for the fusion of vesicles at the plane of cell division.
Gene References into Functions
  1. Immunolocalization of the cytokinesis-specific syntaxin KNOLLE (KN) in ap1m2 showed that KN was mislocalized and aggregated around the division plane. PMID: 23543752
  2. Two distinct types of KNOLLE complexes appear to jointly mediate membrane fusion in Arabidopsis cytokinesis. PMID: 23515225
  3. KEULE interacted with an open conformation of KNOLLE that complemented both knolle and keule mutants. This interaction occurred at the cell division plane and required the KNOLLE linker sequence between helix Hc and SNARE domain. PMID: 22595672
  4. KNOLLE regions are necessary for syntaxin function in cytokinesis. PMID: 21838778
  5. Restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis. PMID: 19959995

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Subcellular Location
Membrane; Single-pass type IV membrane protein.
Protein Families
Syntaxin family
Tissue Specificity
Abundant in flowers and developing siliques. A low level expression is seen in the seedlings, roots, and leaves.
Database Links

KEGG: ath:AT1G08560

STRING: 3702.AT1G08560.1

UniGene: At.10802

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