Tbh Antibody

Code CSB-PA769720XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Tbh Polyclonal antibody
Uniprot No.
Target Names
Tbh
Alternative Names
Tbh; CG1543; Tyramine beta-hydroxylase
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Tbh protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Converts tyramine into octopamine, a neurotransmitter involved in ovulation and locomotion. Functions in an amine-mediated Bacc-dependent signaling pathway that negatively regulates acute ethanol sensitivity.
Gene References into Functions
  1. While octopamine (OA) release reduced body fat, we inferred that tyramine (TA)release has the opposite effect, rescuing the fat deposition phenotype clearly seen in Tbh-deficient Drosophila. PMID: 27759117
  2. Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine beta-monooxygenase. PMID: 23320946
  3. Data show that ovulation is defective in females lacking tyramine beta-hydroxylase, resulting in blockage of mature oocytes within the ovaries. PMID: 14623230
  4. Recombinant tyramine beta-monooxygenase requires copper for activity and has a typical type 2 copper electron spin resonance (EPR) spectrum. PMID: 16376104
  5. Octopamine is not the natural signal for flight initiation because flies lacking octopamine [tyramine-beta-hydroxylase null mutants] can fly, though they show markedly different flight-initiation and -maintenance capabilities compared to wild-type flies. PMID: 17928454
  6. the tyramine beta-monooxygenase mechanism is different from that of the mammalian enzyme, dopamine beta-monooxygenase PMID: 18032384

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Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Copper type II ascorbate-dependent monooxygenase family
Tissue Specificity
Present in head and in neurons innervating the oviduct (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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