Recombinant Drosophila melanogaster Tyramine beta-hydroxylase (Tbh)

Code CSB-CF769720DLU
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Tbh
Uniprot No.
Alternative Names
Tbh; CG1543; Tyramine beta-hydroxylase
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-670
Target Protein Sequence
MLKIPLQLSSQDGIWPARFARRLHHHHQLAYHHHKQEQQQQQQQQQQQQAKQKQKQNGVQ QGRSPTFMPVMLLLLMATLLTRPLSAFSNRLSDTKLHEIYLDDKEIKLSWMVDWYKQEVL FHLQNAFNEQHRWFYLGFSKRGGLADADICFFENQNGFFNAVTDTYTSPDGQWVRRDYQQ DCEVFKMDEFTLAFRRKFDTCDPLDLRLHEGTMYVVWARGETELALEDHQFALPNVTAPH EAGVKMLQLLRADKILIPETELDHMEITLQEAPIPSQETTYWCHVQRLEGNLRRRHHIVQ FEPLIRTPGIVHHMEVFHCEAGEHEEIPLYNGDCEQLPPRAKICSKVMVLWAMGAGTFTY PPEAGLPIGGPGFNPYVRLEVHFNNPEKQSGLVDNSGFRIKMSKTLRQYDAAVMELGLEY TDKMAIPPGQTAFPLSGYCVADCTRAALPATGIIIFGSQLHTHLRGVRVLTRHFRGEQEL REVNRDDYYSNHFQEMRTLHYKPRVLPGDALVTTCYYNTKDDKTAALGGFSISDEMCVNY IHYYPATKLEVCKSSVSEETLENYFIYMKRTEHQHGVHLNGARSSNYRSIEWTQPRIDQL YTMYMQEPLSMQCNRSDGTRFEGRSSWEGVAATPVQIRIPIHRKLCPNYNPLWLKPLEKG DCDLLGECIY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Converts tyramine into octopamine, a neurotransmitter involved in ovulation and locomotion. Functions in an amine-mediated Bacc-dependent signaling pathway that negatively regulates acute ethanol sensitivity.
Gene References into Functions
  1. While octopamine (OA) release reduced body fat, we inferred that tyramine (TA)release has the opposite effect, rescuing the fat deposition phenotype clearly seen in Tbh-deficient Drosophila. PMID: 27759117
  2. Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine beta-monooxygenase. PMID: 23320946
  3. Data show that ovulation is defective in females lacking tyramine beta-hydroxylase, resulting in blockage of mature oocytes within the ovaries. PMID: 14623230
  4. Recombinant tyramine beta-monooxygenase requires copper for activity and has a typical type 2 copper electron spin resonance (EPR) spectrum. PMID: 16376104
  5. Octopamine is not the natural signal for flight initiation because flies lacking octopamine [tyramine-beta-hydroxylase null mutants] can fly, though they show markedly different flight-initiation and -maintenance capabilities compared to wild-type flies. PMID: 17928454
  6. the tyramine beta-monooxygenase mechanism is different from that of the mammalian enzyme, dopamine beta-monooxygenase PMID: 18032384

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Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Copper type II ascorbate-dependent monooxygenase family
Tissue Specificity
Present in head and in neurons innervating the oviduct (at protein level).
Database Links
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