Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TCP4 Polyclonal antibody

Uniprot No.

Q8LPR5

Target Names

TCP4

Alternative Names

TCP4 antibody; MEE35 antibody; At3g15030 antibody; K15M2.17 antibody; Transcription factor TCP4 antibody; Protein MATERNAL EFFECT EMBRYO ARREST 35 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TCP4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor playing a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes such as CUC genes, probably through the induction of miRNA (e.g. miR164). Required during early steps of embryogenesis. Participates in ovule develpment. Activates LOX2 expression by binding to the 5'-GGACCA-3' motif found in its promoter. Activates YUC5 transcription by binding to the 5'-GTGGGCCA-3' motif found in its promoter. Through the activation of YUC5 transcription, integrates the auxin response to a brassinosteroid-dependent molecular circuit that promotes cell elongation in hypocotyls. Activates GIS transcription by binding to the 5'-TGGTCC-3' motif found in its promoter. Involved in the regulation of trichome branching through the activation of GIS transcription. Activates CO transcription by binding to the 5'-GGACCAC-3' motif found in its promoter. Involved in the regulation of photoperiodic flowering through the activation of CO transcription. Activates TCL1 and TCL2 transcription by binding to the 5'-TGGCCA-3' and 5'-GTGGACCA-3' motifS found in their respective promoters. Involved in the suppression of trichome initiaition through the activation of TCL1 and TCL2 transcription. Activates HAT2 transcription by binding to the 5'-TGGTCCAC-3' motif found in its promoter. Through the activation of HAT2 transcription, involved in the auxin-independent reprogramming of mitotic cells to exit division and acquire differentiation competence within the transition zone.

Gene References into Functions

Detailed genetic analyses show that GIS and TCP4 work in same pathway and GIS function is required for TCP4-mediated regulation of trichome differentiation. PMID: 29165850
Mutations in MIR319 and gain-of-function mutations in the TCP4 gene conferred resistance against miR319 and impaired the cotyledon boundary and leaf serration formation. Taken together, these findings demonstrate that the MIR319 and TCP gene families underlie robust and multilayer control of leaf development. PMID: 28842549
miR319 and its target TCP4 can act as switches that turn on secondary cell wall synthesis and programmed cell death. PMID: 28233945
Our yeast one-hybrid analysis revealed that class II CIN-TCPs, including TCP4, bind to the CO promoter. TCP4 induces CO expression around dusk by directly associating with the CO promoter in vivo. In addition, TCP4 binds to another flowering regulator, GIGANTEA (GI), in the nucleus, and induces CO expression in a GI-dependent manner PMID: 28628608
Data showed that SPOROCYTELESS (SPL) most likely functions as a transcription repressor by interacting with TCP family transcription factors and the TOPLESS (TPL) co-repressors. PMID: 25527103
A small increase in TCP4 activity has an immediate impact on leaf cell number, by significantly reducing cell proliferation through miR319. PMID: 25053833
Data show that the expression of TCP4 and IAMT1 that encodes a methyltransferase converting free indole-3-acetic acid (IAA) to methyl-IAA ester (MeIAA) was induced and the induction level was dependent on the abundance of TaWRKY71-1 transcripts. PMID: 23671653
TCP4 protein blocks cell cycle of budding yeast, specifically at G1->S transition, by regulating G1 checkpoint control pathway. PMID: 21658370
These analyses indicate a role for auxin, gibberellic acid and abscisic acid in TCP4-mediated control of leaf growth. PMID: 21904111
hyper-activation of TCP4 revealed its role in determining the timing of crucial developmental events, both at the organ and organism level. PMID: 21518050
a miRNA-resistant version of TCP4, when expressed in petals and stamens, causes these organs not to develop PMID: 20007771
Arabidopsis plants with an increased activity of the transcription factor TCP4, which reduces cell proliferation in leaves, have higher miR396 and lower GRF levels. PMID: 20023165

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in cotyledons, particularly in the vascular region, in leaves, roots, buds, flowers and immature siliques.

Database Links

KEGG: ath:AT3G15030

STRING: 3702.AT3G15030.1

UniGene: At.23764

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TCP4 Antibody

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