Product Details

Purity

>85% (SDS-PAGE)

Target Names

TCP4

Uniprot No.

Q8LPR5

Alternative Names

TCP4; MEE35; At3g15030; K15M2.17; Transcription factor TCP4; Protein MATERNAL EFFECT EMBRYO ARREST 35

Species

Arabidopsis thaliana (Mouse-ear cress)

Expression Region

1-420

Target Protein Sequence

MSDDQFHHPP PPSSMRHRST SDAADGGCGE IVEVQGGHIV RSTGRKDRHS KVCTAKGPRD RRVRLSAHTA IQFYDVQDRL GFDRPSKAVD WLIKKAKTSI DELAELPPWN PADAIRLAAA NAKPRRTTAK TQISPSPPPP QQQQQQQQLQ FGVGFNGGGA EHPSNNESSF LPPSMDSDSI ADTIKSFFPV IGSSTEAPSN HNLMHNYHHQ HPPDLLSRTN SQNQDLRLSL QSFPDGPPSL LHHQHHHHTS ASASEPTLFY GQSNPLGFDT SSWEQQSSEF GRIQRLVAWN SGGGGGATDT GNGGGFLFAP PTPSTTSFQP VLGQSQQLYS QRGPLQSSYS PMIRAWFDPH HHHQSISTDD LNHHHHLPPP VHQSAIPGIG FASGEFSSGF RIPARFQGQE EEQHDGLTHK PSSASSISRH

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcription factor playing a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes such as CUC genes, probably through the induction of miRNA (e.g. miR164). Required during early steps of embryogenesis. Participates in ovule develpment. Activates LOX2 expression by binding to the 5'-GGACCA-3' motif found in its promoter. Activates YUC5 transcription by binding to the 5'-GTGGGCCA-3' motif found in its promoter. Through the activation of YUC5 transcription, integrates the auxin response to a brassinosteroid-dependent molecular circuit that promotes cell elongation in hypocotyls. Activates GIS transcription by binding to the 5'-TGGTCC-3' motif found in its promoter. Involved in the regulation of trichome branching through the activation of GIS transcription. Activates CO transcription by binding to the 5'-GGACCAC-3' motif found in its promoter. Involved in the regulation of photoperiodic flowering through the activation of CO transcription. Activates TCL1 and TCL2 transcription by binding to the 5'-TGGCCA-3' and 5'-GTGGACCA-3' motifS found in their respective promoters. Involved in the suppression of trichome initiaition through the activation of TCL1 and TCL2 transcription. Activates HAT2 transcription by binding to the 5'-TGGTCCAC-3' motif found in its promoter. Through the activation of HAT2 transcription, involved in the auxin-independent reprogramming of mitotic cells to exit division and acquire differentiation competence within the transition zone.

Gene References into Functions

Detailed genetic analyses show that GIS and TCP4 work in same pathway and GIS function is required for TCP4-mediated regulation of trichome differentiation. PMID: 29165850
Mutations in MIR319 and gain-of-function mutations in the TCP4 gene conferred resistance against miR319 and impaired the cotyledon boundary and leaf serration formation. Taken together, these findings demonstrate that the MIR319 and TCP gene families underlie robust and multilayer control of leaf development. PMID: 28842549
miR319 and its target TCP4 can act as switches that turn on secondary cell wall synthesis and programmed cell death. PMID: 28233945
Our yeast one-hybrid analysis revealed that class II CIN-TCPs, including TCP4, bind to the CO promoter. TCP4 induces CO expression around dusk by directly associating with the CO promoter in vivo. In addition, TCP4 binds to another flowering regulator, GIGANTEA (GI), in the nucleus, and induces CO expression in a GI-dependent manner PMID: 28628608
Data showed that SPOROCYTELESS (SPL) most likely functions as a transcription repressor by interacting with TCP family transcription factors and the TOPLESS (TPL) co-repressors. PMID: 25527103
A small increase in TCP4 activity has an immediate impact on leaf cell number, by significantly reducing cell proliferation through miR319. PMID: 25053833
Data show that the expression of TCP4 and IAMT1 that encodes a methyltransferase converting free indole-3-acetic acid (IAA) to methyl-IAA ester (MeIAA) was induced and the induction level was dependent on the abundance of TaWRKY71-1 transcripts. PMID: 23671653
TCP4 protein blocks cell cycle of budding yeast, specifically at G1->S transition, by regulating G1 checkpoint control pathway. PMID: 21658370
These analyses indicate a role for auxin, gibberellic acid and abscisic acid in TCP4-mediated control of leaf growth. PMID: 21904111
hyper-activation of TCP4 revealed its role in determining the timing of crucial developmental events, both at the organ and organism level. PMID: 21518050
a miRNA-resistant version of TCP4, when expressed in petals and stamens, causes these organs not to develop PMID: 20007771
Arabidopsis plants with an increased activity of the transcription factor TCP4, which reduces cell proliferation in leaves, have higher miR396 and lower GRF levels. PMID: 20023165

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in cotyledons, particularly in the vascular region, in leaves, roots, buds, flowers and immature siliques.

Database Links

KEGG: ath:AT3G15030

STRING: 3702.AT3G15030.1

UniGene: At.23764

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Recombinant Arabidopsis thaliana Transcription factor TCP4 (TCP4)

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