TFC4 Antibody

Code CSB-PA329250XA01SVG
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: TFC4 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54.9 kDa
    Observed band size: 61 kDa
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TFC4 Polyclonal antibody
Uniprot No.
Target Names
TFC4
Alternative Names
TFC4 antibody; PCF1 antibody; YGR047C antibody; Transcription factor tau 131 kDa subunit antibody; TFIIIC 131 kDa subunit antibody; Transcription factor C subunit 4 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TFC4 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

An immune response is induced in a rabbit injected with the recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TFC4 protein. Antibodies against the TFC4 protein are generated by the rabbit's immune system over time. After a sufficient antibody response is produced, a rabbit serum sample is collected to obtain polyclonal antibodies. The TFC4 antibody undergoes protein A/G affinity chromatography purification. The effectiveness of the TFC4 antibody is assessed in ELISA and WB applications. This specific TFC4 antibody can react with the Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TFC4 protein.

The Saccharomyces cerevisiae TFC4 protein helps in the assembly and stability of the TFIIIC complex, allowing it to bind to specific DNA sequences and recruit RNA polymerase III for transcription. TFIIIC is involved in the initiation of transcription by RNA polymerase III, which is responsible for transcribing genes involved in the production of various types of RNA molecules such as tRNA and rRNA.

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Target Background

Function
TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. TFC4 is the TFIIIB-assembling subunit of TFIIIC and essential for viability.
Gene References into Functions
  1. The data are consistent with this peptide causing a conformational change in TFIIIC that overcomes Tfc4 autoinhibition of Brf1 binding and suggest a structural model for the Brf1-Tfc4 interaction. PMID: 16880507
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YGR047C

STRING: 4932.YGR047C

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