TFL1 Antibody

Code CSB-PA310106XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TFL1 Polyclonal antibody
Uniprot No.
Target Names
TFL1
Alternative Names
TFL1 antibody; At5g03840 antibody; F8F6_50 antibody; Protein TERMINAL FLOWER 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TFL1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Controls inflorescence meristem identity and is required for maintenance of an indeterminate inflorescence. Prevents the expression of 'APETALA1' and 'LEAFY'. Also plays a role in the regulation of the time of flowering in the long-day flowering pathway. May form complexes with phosphorylated ligands by interfering with kinases and their effectors.
Gene References into Functions
  1. TFL1 can act outside of its normal expression domain in leaf primordia or floral meristems to repress flower identity. These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture. PMID: 26019254
  2. the expression of Dt2 is unable to repress TFL1 in Arabidopsis, further demonstrating the evolutionary novelty of the regulatory mechanism underlying stem growth in soybean. PMID: 26807727
  3. conserved and diverse functions of the FT/TFL1 gene PMID: 25598141
  4. The results identify AGO1 as a repressor of TFL1 expression. AGO1 plays a role in inflorescence development, controlling the production of coflorescences. AGO1 seems to play this role through regulating TFL1 expression. PMID: 24989784
  5. The balance of the FT and TFL1 gene expression is important for determining flowering behavior. PMID: 23543784
  6. common bean growth habit gene PvTFL1y is a functional homolog of Arabidopsis TFL1 PMID: 22331140
  7. TFL1 negatively modulates the FD-dependent transcription of target genes to fine-tune flowering time and the development of the inflorescence meristem. PMID: 21890645
  8. A region important in vivo localizes to a 14-amino-acid segment that evolves very rapidly in TFL1 orthologs, but is almost invariant in FT orthologs. PMID: 16424903
  9. TFL1 gene plays an important role in hormone-regulated inflorescence formation. PMID: 16676184
  10. Signals from LFY in floral meristems may feed back to promote TFL1 protein movement in the shoot meristem. PMID: 17369370
  11. TFL1 localizes to endomembrane compartments and colocalizes with the putative delta-subunit of the AP-3 adapter complex PMID: 18003908
  12. The activation of TFL1 expression by PPF1 defines an important pathway possibly essential for the negative regulation of plant senescence in transgenic Arabidopsis. PMID: 18713382

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Subcellular Location
Cytoplasm.
Protein Families
Phosphatidylethanolamine-binding protein family
Tissue Specificity
Expressed below the apical dome of inflorescence and coflorescence meristems, and in inflorescence stem.
Database Links

KEGG: ath:AT5G03840

STRING: 3702.AT5G03840.1

UniGene: At.1041

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