Recombinant Arabidopsis thaliana Protein TERMINAL FLOWER 1 (TFL1)

Code CSB-YP310106DOA
MSDS
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Source Yeast
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Code CSB-EP310106DOA
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Source E.coli
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Code CSB-EP310106DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP310106DOA
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Source Baculovirus
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Code CSB-MP310106DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TFL1
Uniprot No.
Alternative Names
TFL1; At5g03840; F8F6_50; Protein TERMINAL FLOWER 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-177
Target Protein Sequence
MENMGTRVIE PLIMGRVVGD VLDFFTPTTK MNVSYNKKQV SNGHELFPSS VSSKPRVEIH GGDLRSFFTL VMIDPDVPGP SDPFLKEHLH WIVTNIPGTT DATFGKEVVS YELPRPSIGI HRFVFVLFRQ KQRRVIFPNI PSRDHFNTRK FAVEYDLGLP VAAVFFNAQR ETAARKR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Controls inflorescence meristem identity and is required for maintenance of an indeterminate inflorescence. Prevents the expression of 'APETALA1' and 'LEAFY'. Also plays a role in the regulation of the time of flowering in the long-day flowering pathway. May form complexes with phosphorylated ligands by interfering with kinases and their effectors.
Gene References into Functions
  1. TFL1 can act outside of its normal expression domain in leaf primordia or floral meristems to repress flower identity. These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture. PMID: 26019254
  2. the expression of Dt2 is unable to repress TFL1 in Arabidopsis, further demonstrating the evolutionary novelty of the regulatory mechanism underlying stem growth in soybean. PMID: 26807727
  3. conserved and diverse functions of the FT/TFL1 gene PMID: 25598141
  4. The results identify AGO1 as a repressor of TFL1 expression. AGO1 plays a role in inflorescence development, controlling the production of coflorescences. AGO1 seems to play this role through regulating TFL1 expression. PMID: 24989784
  5. The balance of the FT and TFL1 gene expression is important for determining flowering behavior. PMID: 23543784
  6. common bean growth habit gene PvTFL1y is a functional homolog of Arabidopsis TFL1 PMID: 22331140
  7. TFL1 negatively modulates the FD-dependent transcription of target genes to fine-tune flowering time and the development of the inflorescence meristem. PMID: 21890645
  8. A region important in vivo localizes to a 14-amino-acid segment that evolves very rapidly in TFL1 orthologs, but is almost invariant in FT orthologs. PMID: 16424903
  9. TFL1 gene plays an important role in hormone-regulated inflorescence formation. PMID: 16676184
  10. Signals from LFY in floral meristems may feed back to promote TFL1 protein movement in the shoot meristem. PMID: 17369370
  11. TFL1 localizes to endomembrane compartments and colocalizes with the putative delta-subunit of the AP-3 adapter complex PMID: 18003908
  12. The activation of TFL1 expression by PPF1 defines an important pathway possibly essential for the negative regulation of plant senescence in transgenic Arabidopsis. PMID: 18713382

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Subcellular Location
Cytoplasm.
Protein Families
Phosphatidylethanolamine-binding protein family
Tissue Specificity
Expressed below the apical dome of inflorescence and coflorescence meristems, and in inflorescence stem.
Database Links

KEGG: ath:AT5G03840

STRING: 3702.AT5G03840.1

UniGene: At.1041

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