Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) THI1 Polyclonal antibody

Uniprot No.

Q38814

Target Names

THI1

Alternative Names

THI1 antibody; ARA6 antibody; THI4 antibody; At5g54770 antibody; MBG8.3 antibody; Thiamine thiazole synthase antibody; chloroplastic antibody; EC 2.4.2.60 antibody; Thiazole biosynthetic enzyme antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana THI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. Acts as a positive regulator for the abscisic acid-induced activation of slow type anion channels during stomatal closure by repressing CPK33 kinase activity.

Gene References into Functions

Consistent with their contrasting regulatory role over stomatal closure, THI1 suppressed CPK33 kinase activity in vitro. PMID: 26662273
The THI1 alanine to valine substitution directly affects the rate of the thiazole biosynthesis. PMID: 24637331
THI1 is targeted to both chloroplasts and mitochondria and is expressed in both shoots and roots during vegetative growth but more active in shoot tissues of mature plants. Expression is affected by high salinity, sugar deprivation and flooding. PMID: 15897230
A point mutation in the thi1 gene, involved in the synthesis of thiamin is responsible for the tz phenotype where the cotyledon is green but the leaves are white, and the plants die early during development. PMID: 12941878
analysis of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana PMID: 16912043

Hide All

Subcellular Location

Plastid, chloroplast. Mitochondrion. Cell membrane.

Protein Families

THI4 family

Tissue Specificity

Expressed at high levels in chloroplast-containing parenchymatic cells of leaves, inflorescence shoots and flowers, and at lower levels in the vascular system. In young plants, detected in roots and shoots including cotyledons, leaves and hypocotyls. Also

Database Links

KEGG: ath:AT5G54770

STRING: 3702.AT5G54770.1

UniGene: At.24888

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA656429XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

THI1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background