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Recombinant Arabidopsis thaliana Thiamine thiazole synthase, chloroplastic (THI1)

Recombinant Arabidopsis thaliana Thiamine thiazole synthase, chloroplastic (THI1)

Code	CSB-YP656429DOA
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP656429DOA
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP656429DOA-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP656429DOA
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP656429DOA
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

THI1

Uniprot No.

Q38814

Alternative Names

THI1; ARA6; THI4; At5g54770; MBG8.3; Thiamine thiazole synthase; chloroplastic; EC 2.4.2.60; Thiazole biosynthetic enzyme

Species

Arabidopsis thaliana (Mouse-ear cress)

Expression Region

56-349

Target Protein Sequence

FTFDP IKESIVSREM TRRYMTDMIT YAETDVVVVG AGSAGLSAAY EISKNPNVQV AIIEQSVSPG GGAWLGGQLF SAMIVRKPAH LFLDEIGVAY DEQDTYVVVK HAALFTSTIM SKLLARPNVK LFNAVAAEDL IVKGNRVGGV VTNWALVAQN HHTQSCMDPN VMEAKIVVSS CGHDGPFGAT GVKRLKSIGM IDHVPGMKAL DMNTAEDAIV RLTREVVPGM IVTGMEVAEI DGAPRMGPTF GAMMISGQKA GQLALKALGL PNAIDGTLVG NLSPELVLAA ADSAETVDA

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. Acts as a positive regulator for the abscisic acid-induced activation of slow type anion channels during stomatal closure by repressing CPK33 kinase activity.

Gene References into Functions

Consistent with their contrasting regulatory role over stomatal closure, THI1 suppressed CPK33 kinase activity in vitro. PMID: 26662273
The THI1 alanine to valine substitution directly affects the rate of the thiazole biosynthesis. PMID: 24637331
THI1 is targeted to both chloroplasts and mitochondria and is expressed in both shoots and roots during vegetative growth but more active in shoot tissues of mature plants. Expression is affected by high salinity, sugar deprivation and flooding. PMID: 15897230
A point mutation in the thi1 gene, involved in the synthesis of thiamin is responsible for the tz phenotype where the cotyledon is green but the leaves are white, and the plants die early during development. PMID: 12941878
analysis of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana PMID: 16912043

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Subcellular Location

Plastid, chloroplast. Mitochondrion. Cell membrane.

Protein Families

THI4 family

Tissue Specificity

Expressed at high levels in chloroplast-containing parenchymatic cells of leaves, inflorescence shoots and flowers, and at lower levels in the vascular system. In young plants, detected in roots and shoots including cotyledons, leaves and hypocotyls. Also

Database Links

KEGG: ath:AT5G54770

STRING: 3702.AT5G54770.1

UniGene: At.24888

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