TIC40 Antibody

Code CSB-PA884298XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TIC40 Polyclonal antibody
Uniprot No.
Target Names
TIC40
Alternative Names
TIC40; PDE120; At5g16620; MTG13.6; Protein TIC 40, chloroplastic; Protein PIGMENT DEFECTIVE EMBRYO 120; Translocon at the inner envelope membrane of chloroplasts 40; AtTIC40
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TIC40 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in protein precursor import into chloroplasts. Part of the motor complex consisting of a co-chaperone (TIC40) and a chaperone (HSP93) associated with the import channel (TIC110). Causes the release of bound transit peptides from TIC110 and stimulates ATP hydrolysis by HSP93. Involved in reinsertion of proteins from the chloroplast stroma into the inner membrane.
Gene References into Functions
  1. Tic40 is a multifunctional protein that, in addition to its originally described role in protein import, is able to influence downstream processes leading to thylakoid biogenesis. PMID: 28684427
  2. Interaction of Tic40-NP and Hsp93 stimulates ATP hydrolysis of Hsp93, but the hydrolysis is abolished in both N320A and N329A mutants of Tic40-NP. PMID: 22888115
  3. The interaction of Tic110, Tic40 and Hsp93 in A. thaliana chloroplast protein transport is described. [AtTic40] PMID: 15659100
  4. The interaction of Tic40 with rhomboid proteases in a yeast mitochondria-based assay is reported. PMID: 17327256
  5. analysis of similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein PMID: 17535810
  6. the sorting of AtTic40 requires a bipartite transit peptide, which is first cleaved by the stromal processing peptidase (SPP), thus generating a soluble AtTic40 stromal intermediate. PMID: 17883373
  7. In a Tic40 mutant soluble Tic40 and Tic110N intermediates accumulated in the chloroplast stroma due to a slower rate of reinsertion into the inner membrane. PMID: 18657235

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Subcellular Location
Plastid, chloroplast inner membrane; Single-pass membrane protein.
Tissue Specificity
Expressed in seedlings, flowers, leaves, stems and roots.
Database Links

KEGG: ath:AT5G16620

STRING: 3702.AT5G16620.1

UniGene: At.25049

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