TIM17 Antibody

Code CSB-PA331035XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM17 Polyclonal antibody
Uniprot No.
Target Names
TIM17
Alternative Names
TIM17; MIM17; MPI2; SMS1; YJL143W; J0648; Mitochondrial import inner membrane translocase subunit TIM17; Mitochondrial inner membrane protein MIM17; Mitochondrial protein import protein 2
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM17 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Gene References into Functions
  1. Mutations in TM1 and TM2 impaired the interaction of Tim17 with Tim23, component of the translocation channel, whereas mutations in TM3 compromised binding of the import motor. The authors identified residues in the matrix-facing region of Tim17 involved in binding of the import motor. PMID: 28165323
  2. charged residues in Tim17 are critical for the preprotein-induced gating of the TIM23 translocase PMID: 15618217
  3. Tim17p is required for twin pore structure and provides the voltage gate for the TIM23 channel complex. PMID: 17148445
  4. Overexpression of TIM17 prevents the complete loss of mtDNA in cells lacking Abf2. PMID: 18826960
Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Protein Families
Tim17/Tim22/Tim23 family
Database Links

KEGG: sce:YJL143W

STRING: 4932.YJL143W

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