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Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM17 (TIM17), partial

Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM17 (TIM17), partial

Code	CSB-YP331035SVG1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP331035SVG1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP331035SVG1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP331035SVG1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP331035SVG1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

TIM17

Uniprot No.

P39515

Alternative Names

TIM17; MIM17; MPI2; SMS1; YJL143W; J0648; Mitochondrial import inner membrane translocase subunit TIM17; Mitochondrial inner membrane protein MIM17; Mitochondrial protein import protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Gene References into Functions

Mutations in TM1 and TM2 impaired the interaction of Tim17 with Tim23, component of the translocation channel, whereas mutations in TM3 compromised binding of the import motor. The authors identified residues in the matrix-facing region of Tim17 involved in binding of the import motor. PMID: 28165323
charged residues in Tim17 are critical for the preprotein-induced gating of the TIM23 translocase PMID: 15618217
Tim17p is required for twin pore structure and provides the voltage gate for the TIM23 channel complex. PMID: 17148445
Overexpression of TIM17 prevents the complete loss of mtDNA in cells lacking Abf2. PMID: 18826960

Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Protein Families

Tim17/Tim22/Tim23 family

Database Links

KEGG: sce:YJL143W

STRING: 4932.YJL143W

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