Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM22 Polyclonal antibody

Uniprot No.

Q12328

Target Names

TIM22

Alternative Names

TIM22; YDL217C; D0884; Mitochondrial import inner membrane translocase subunit TIM22

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM22 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins, such as mitochondrial carrier family members, into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps. Mediates the insertion of precursor proteins in a 3 step process. After the precursor is tethered to the translocase without losing energy from the Delta(psi), 2 energy-requiring steps are needed. First, Delta(psi) acts on the precursor protein and promotes its docking in the translocase complex. Then, Delta(psi) and an internal signal peptide together induce rapid gating transitions in one pore and closing of the other pore and drive membrane insertion to completion.

Gene References into Functions

reduced phosphatidylcholine levels differentially affect the TIM22 and TIM23 complexes in mitochondrial protein transport. PMID: 27402832
We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria. PMID: 23283984
When Hot13p is overexpressed, it may function to remodel the small TIM complexes during import. PMID: 15294910
the carboxyl-terminal module of dicarboxylate carrier possesses major targeting information to facilitate the direct binding of the carrier protein to TIM22 PMID: 15591051
Phosphate carrier (PiC) can be sorted to either the TIM22 pathway or the TIM23 pathway for its import into yeast mitochondria PMID: 15644337
The Tim21 structure represents a new alpha/beta-mixed protein fold with two alpha-helices flanked by an extended eight-stranded beta-sheet. Here we suggest a mechanism for the TOM-TIM interaction. PMID: 17099692
TIM22 as a dynamic channel solely active in the presence of its cargo proteins PMID: 17462993

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Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein. Note=Import into inner membrane protein requires TOM20 function.

Protein Families

Tim17/Tim22/Tim23 family

Database Links

KEGG: sce:YDL217C

STRING: 4932.YDL217C

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TIM22 Antibody

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